Top 41 How To Prepare Methylene Blue Indicator The 59 Latest Answer

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Methylene Blue Indicator Solution: Dissolve 150 mg of methylene blue in 100 ml of ethanol (95 percent) and dilute with ethanol (95 percent) to produce 250 ml.Methylene blue stain: Prepare a stock solution by adding 1.0 g methylene blue chloride to 10 mL 95% ethyl alcohol and slowly adding 100 mL distilled water and 5 mL phenol (melted crystals).Methylene blue is a good redox reaction indicator: it’s blue when mixed with an oxidising agent, but turns colourless when mixed with a reducing agent. One of the traditional ways of demonstrating chemical kinetics in general chemistry is the blue bottle experiment.

Add 5 g to 500 mL of ethanol, add 500 mL water. Stir.
Dissolve 1 g of phenolphthalein powder in 500 mL of 50% alcohol. Add drops of this phenolphthalein solution to 100 mL of 0.5 mol / litre sodium hydroxide solution until a deep pink colour appears. …
Add colourless phenolphthalein indicator to lime water.

Contents

How do you make methylene blue?

Methylene blue stain: Prepare a stock solution by adding 1.0 g methylene blue chloride to 10 mL 95% ethyl alcohol and slowly adding 100 mL distilled water and 5 mL phenol (melted crystals).

How do you make an indicator?

Add 5 g to 500 mL of ethanol, add 500 mL water. Stir.
Dissolve 1 g of phenolphthalein powder in 500 mL of 50% alcohol. Add drops of this phenolphthalein solution to 100 mL of 0.5 mol / litre sodium hydroxide solution until a deep pink colour appears. …
Add colourless phenolphthalein indicator to lime water.

What is the indicator of methylene blue?

Methylene blue is a good redox reaction indicator: it’s blue when mixed with an oxidising agent, but turns colourless when mixed with a reducing agent. One of the traditional ways of demonstrating chemical kinetics in general chemistry is the blue bottle experiment.

How do you dissolve methylene blue?

Dissolve 5.0 g of methylene blue powder in 450 mL of purified water by heating 30 mins.

Is methylene blue basic or acidic?

Methylene blue (C.I. 52015; Basic blue 9) is a basic thiazine dye. It may have more scientific uses than any other dye. As a simple stain, applied from a mildly acidic solution (pH 3 to 4) it colors nucleic acids and acidic carbohydrates.

How do you prepare methyl indicator?

Methyl Red Indicator Solution: Dissolve 50 mg of methyl red in a mixture of 1.86 ml of 0.1 M sodium hydroxide and 50 ml of ethanol (95 percent). After the solution is effected, add sufficient water to produce 100 ml. Complies with the following test.

What is the pH range of methyl red?

Methyl red (pH range: 4.4–6.2) is an azo compound, the structure of which differs from methyl orange (Orange 3) one by the substitution of sulphonic acid function by carboxylic acid function (Fig. 3.19).

Is methylene blue a pH indicator?

Tashiro’s indicator is a pH indicator (pH value: 4.4–6.2), mixed indicator composed of a solution of methylene blue (0.1%) and methyl red (0.03%) in ethanol or in methanol.
…
Tashiro’s indicator.

Tashiro’s indicator (pH indicator)
below pH 4.4		above pH 6.2
4.4	⇌	6.2

How do you stain methylene blue?

Ⅴ. How to stain with methylene blue

After performing the bacterial smear (smearing, fixing)
Place the slide on a staining rack and flood it with methylene blue. …
Gently wash the slide with distilled water, drain off excess water, blot (do not rub) with absorbent paper and allow slides to air dry completely.

Should I dilute methylene blue?

Methylene Blue Injection

A suitable dilution for oral dosing would be 5-10 mL of the 1% solution diluted to 100-200 mL with water for injections. The high volume is suggested to reduce the degree of gastrointestinal disturbances and dysuria.

Is methylene blue stable in water?

MB is an aromatic heterocyclic basic dye [19] having a molecular weight of 319.85 g mol⁻¹ [20,21]. MB is a well-known cationic and primary thiazine dye with a molecular formula- C₁₆H₁₈N₃ClS, having λ_max of 663 nm. It is highly water-soluble, and thus forms a stable solution with water at room temperature [22,23,24,25].

Does ethanol dissolve methylene blue?

The solubility of methylene blue in methanol, ethanol, 2-propanol, acetone, ethyl acetate, and water was measured at 303.15 K and 0.1 MPa. The solubility of methylene blue in ethyl acetate was very low while the solubility in methanol and 2-propanol was very high and not precisely measurable.

What is methylene blue made from?

Methylene blue is synthesized commercially by oxidation of N,N-dimethyl-phenylenediamine with sodium dichromate (Na2Cr2O7) in the presence of sodium thiosulfate (Na2S2O3), followed by further oxidation in the presence of N,N-dimethylaniline (NTP, 2008).

How do you make methylene blue solution for fish?

Add 1 teaspoon of 2.303% Methylene Blue per 10 gallons of water. This produces a concentration of 3 ppm. For increased concentrations, add approximately 1/3 teaspoon (1.64 ml) per 10 gallons for each required 1 ppm increase. Only one application is needed.

What is methylene blue used for?

Descriptions. Methylene blue injection is used to treat a condition called methemoglobinemia. This condition occurs when the blood cannot deliver oxygen where it is needed in the body. This medicine is to be given only by or under the supervision of a doctor.

Is methylene blue poisonous?

Methylene blue is a safe drug when used in therapeutic doses (<2mg/kg). But it can cause toxicity in high doses.

how to prepare methyl blue indicator | how to make methyl blue indicator

Preparation of Indicator Solutions : Pharmaguideline

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What is the Purpose of Methylene Blue?

Methylene blue has several medical applications, such as treating the blood disorder methemoglobinemia and counteracting the poisonous effects of potassium cyanide. In chemistry, it’s one of the various types of chemical indicators used in titration experiments and similar analytical methods. It’s used as a redox indicator, peroxide generator, water titrant, and sulphide indicator.

What is the Chemical Name for Methylene Blue?

Methylene blue is known by many different names, most commonly methylthioninium chloride, but also Urelene blue, Provayblue, and Proveblue. Its IUPAC (International Union of Pure and Applied Chemistry) standardised name is 7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride. Since this is descriptive, we can use it to deduce the chemical formula and chemical structure.

The name, specifically the chloride part, tells us that there is a presence of chlorine. However, the methylene group is structurally complex, and while we know it also contains sulphur and nitrogen, it would take an advanced knowledge of biochemistry to figure out the rest of the chemical composition solely from the IUPAC name.

Methylene blue is a derivative of phenothiazine, but is prepared by the oxidation of dimethyl-4-phenylenediamine in the presence of sodium thiosulfphte. The chemical equation for the reaction is shown below. As you can see, the sulfur is derived from sodium thiosulfate:

What is the Chemical Formula of Methylene Blue?

Methylene blue is a relatively complex organic compound that has three cyclic structures attached in a chain. Its chemical formula can be written simply as C 16 H 18 ClN 3 S. It has a molar mass of 319.85 g·mol−1 and becomes slightly acidic when dissolved in water.

But the molecular structure of the compound is actually a bit more nuanced, as shown in the illustration below. As you can see, sulphur and nitrogen are attached to the central ring, while chlorine is a separate group and is negatively charged:

What is the Function of Methylene Blue?

Methylene blue is a type of salt, meaning it has ionic bonds and a crystalline structure. As a salt, it dissociates as ions in water and turns the solution slightly acidic. While it mainly functions as a dye, it has other properties that make it suitable as a chemical indicator as well as a medication.

As a dye, methylene blue is very useful in the laboratory as an indicator of chemical change and as a biological stain for bacterial cells that are examined under the microscope. The dye highlights the contrasts, revealing details of the cells. The dye then attaches to protein receptors, allowing it to highlight the shape and structure of the cells.

These same chemical properties, which is having an affinity to stain cells, also make this salt useful as a medication and diagnostic tool in detecting certain abnormalities, like precancerous cells. While we know it has selective dye uptake effects on certain types of tissues, like nerve fibres, the exact mechanism isn’t yet fully understood.

What is Methylene Blue Used For?

Methylene blue is primarily used as a dye, but its dye properties also allow it to be useful in several other applications, such as in medicine, and in chemistry as a chemical indicator for various types of chemical reactions or properties.

Methemoglobinemia Treatment

Methemoglobinemia is a medical condition where methemoglobin is elevated in the blood. It’s a form of metalloprotein that’s similar to hemoglobin, but with an iron in the heme group that’s in the ferric (Fe3+) state, unlike the normal hemoglobin’s heme group, which is in the ferrous (Fe2+) state. The abnormal hemoglobin cannot bind with oxygen, which results in oxygen deficiency.

This condition is oftentimes caused by the ingestion of certain pharmaceuticals, toxins, or broad beans. Some of the symptoms include the following, all of which are due to insufficient oxygen levels in the blood:

Headache

Dizziness

Difficulty breathing

Nausea

Muscle coordination problems

Blue-coloured skin or cyanosis

Seizures

Heartbeat irregularities

Methylene blue and oxygen therapy is used to treat methemoglobinemia. The chemical pathway is through NADH or NADPH dependent methemoglobin reductase enzymes. The abnormal hemoglobin is reduced back to normal hemoglobin. However, high doses of this salt could also induce methemoglobinemia, meaning proper dosage is critical.

Redox Indicator

Redox, or oxidation-reduction reactions, involve the transfer of electrons from one species to another. When a chemical species is oxidised, it loses electrons but gains or combines with oxygen. Conversely, reduction is the gaining of electrons and the losing of oxygen. Redox reactions always go side-by-side. An example of this is the oxidation-reduction reaction between ferric oxide and carbon monoxide, as shown in the balanced chemical equation below:

Methylene blue is a good redox reaction indicator: it’s blue when mixed with an oxidising agent, but turns colourless when mixed with a reducing agent.

One of the traditional ways of demonstrating chemical kinetics in general chemistry is the blue bottle experiment. In this classical experiment, a solution is made from glucose, methylene blue, and sodium hydroxide. The solution container, typically a flask, is shaken, resulting in the oxidation of methylene blue. This turns the solution blue. The glucose then gradually reduces the chemical indicator, turning it colourless. The solution will again turn blue as the glucose is consumed in the reaction.

Watch a demonstration of this experiment:

Cyanide Antidote

The reduction capacity of methylene blue is very similar to that of oxygen, in that it can be reduced by components of the electron transport chain. Therefore, it can be used as an antidote for potassium cyanide poisoning. The method for this was first proven effective in 1933 by Dr. Matilda Moldenhauer Brooks.

Water Testing

Methylene blue can also be used to detect surfactants in water samples. For example, you could make a solution of methylene blue and chloroform in order to determine the presence of anionic surfactants, such as traces of detergents or emulsifiers.

What Are the Chemical Properties of Methylene Blue?

The key chemical properties of methylene blue are its colour changing ability and the fact that it becomes acidic when dissolved in water.

In a solution, methylene blue is slightly acidic and dissociates into its ionic constituents. An aqueous solution of this compound has a pH level of 6 at a concentration of 10g/l and at an ideal temperature of 25 °C.

Meanwhile, the colour change in methylene blue is because it loses electrons in the presence of oxidising agents. This makes it good at detecting oxygen in a solution.

Methylene Blue Indicator 0.1% Solution

Reagecon’s Methylene Blue Indicator 0.1% Solution is a high quality Indicator Solution produced from the highest quality raw materials and manufactured and tested under rigorous conditions. For manual titrations; indicator titrant or analyte change colour and this colour change is by far the most important method of end point detection, in such titrations. A high quality indicator solution such as Reagecon’s Methylene Blue Indicator 0.1% Solution facilitates end point detection, in a clear and unambiguous manner and is an imperative for accurate manual titrimetry.

Methylene Blue

Methylene blue is a colorful organic chloride salt compound used in medicine and by biologists as a dye to help them see under the microscope. It is also known as Methylthioninium chloride or Swiss blue. It is represented by the formula \[C_{16}H_{18}CIN_{3}S\]. It was discovered in the year 1876 by German chemist Heinrich Caro. Historically, it has been widely used in Africa for treating malaria, but now it has been disapproved when chloroquine and other drugs entered the market. Its molecular weight is 319.85 g/mol. It can cause damage to RBCs and also decreases the ability of the blood to carry oxygen which is the primary health concern of humans when exposed to methylene blue via oral or intravenous means. Methylene blue is a thiazine dye that is basic in nature. Methylene blue stains negatively charged cell components like nucleic acids.

Structure of Methylene Blue

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Structure of Methylene Blue\[C_{16}H_{18}CIN_{3}S\]
Formula of Methylene Blue

Methylene blue is represented by the formula \[C_{16}H_{18}CIN_{3}S\]. It has 3,7-bis(dimethylamino) phenothiazine – 5 – ium as the counterion. Its IUPAC name is

7−(dimethylamino)phenothiazin−3−ylidene

7−(dimethylamino)phenothiazin−3−ylidene – methyl azanium; chloride.

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Preparation of Methylene Blue

Methylene blue is prepared by the oxidation of dimethyl-4-phenylenediamine in the presence of sodium thiosulfate.

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By treating the solution of N, N-dimethyl-p-phenylenediamine and N, N, -dimethylaniline hydrochlorides with \[H_{2}S\] and \[FeCl_{3}\] or another suitable oxidizing agent. By oxidation of p- amino dimethyl aniline with ferric chloride in the presence of hydrogen sulphide. The dye is zinc chloride double salt of the chloride. N, N-Dimethyl – p – phenylenediamine reacts with sodium thiosulfate to produce thiosulfuric acid which then condenses with N, N-dimethylaniline when it comes in the presence of \[Na_{2}Cr_{2}O_{7}\] or sodium dichromate to form the indamine, then with copper sulphate and sodium dichromate to the methylene blue.

Related Compounds to Methylene Blue

Methylene Blue cation

Azure A

Azure B

Methylene green

3-(Dimethylamino)-7-(methylamino)phenothiazin-5-ium

3,7-Bis(dimethylamino)-4-nitro phenothiazine-5-ium

Properties of Methylene Blue

Methylene blue is deep blue in colour.

Methylene blue exhibits antioxidant properties.

It also exhibits the property of antidepressants.

It is highly soluble in water, chloroform, ethanol, and glacial acetic acid.

It is insoluble in ethyl ether, xylene, and oleic acid.

It also has the property of being antimalarial.

It gets decomposed while heating and emits toxic fumes of nitrogen oxides, sulphur oxides, and chlorides.

It is non-carcinogenic in nature.

It is slightly soluble in pyridine.

It is basic in nature. It’s a basic thiazine dye.

It also exhibits cardioprotective properties.

It is non-combustible in nature.

It acts as the enzyme inhibitor.

What is the Role of Methylene Blue in the Field of Chemistry?

Methylene blue is widely used in the field of chemistry as a redox indicator in analytical chemistry, that is, to detect the presence or absence of oxygen and other oxidising agents due to the change in colour of the solution to blue when an electron is taken from methylene blue. Also, methylene blue is further used in the analysis of volumetric titrations and in Fehling’s test.

What is the Role of Methylene Blue in the Field of Biology?

Apart from chemistry, methylene blue is also used by biologists in various biological experiments as a stain, for example, for identification and visualisation of bacteria, for identification of nucleic acids, that is, DNA and RNA, or in the techniques of northern blotting or as an alternative to crystal violet chemical and ethidium bromide used in the technique of western blotting. Apart from this, methylene blue is also used to calculate the percentage of viable cells in a yeast sample.

What is the Role of Methylene Blue in the Field of Aquaculture?

The use of methylene blue is not restricted to the only chemical and medicinal field but has also been proved to be very useful in the field of aquaculture for the prevention of bacterial and fungal infections on the eggs of freshwater fish. It also increases the oxygen-carrying capacity of fish’s hemoglobin or for the treatment of fish infected with parasitic protozoan.

Uses of Methylene Blue

Methylene blue is used in aqua farming for treating fungal infections.

It is used as an antiseptic in cases of urinary tract infections.

It is also used as an indicator during the different chemical reactions.

It is also used for the treatment of Methemoglobinemia.

It is also used to dye paper and office supplies, but also to tone up silk colours.

It is also used in the treatment of septic shock and anaphylaxis.

It is also used as a dye for a number of staining procedures.

It is also used as a temporary hair colorant.

It is used as an oxidation-reduction agent in different chemical reactions.

It is used in the treatment of pediatric and adult patients with methemoglobinemia.

It is used as a reagent in oxidation-reduction titrations in volumetric analysis.

It is used in the treatment of hypoxia.

It is used for dyeing cotton and wool.

Earlier methylene blue was used to check whether the pasteurized milk is bacteria-free or not. If the colour remains blue, that means milk is pasteurized properly, or else if blue colour disappears that means bacteria is consuming oxygen and the milk is not pasteurized properly.

What are the Warnings Associated with Methylene Blue when it is Used as a Component of a Drug?

Though methylene blue is widely used as a drug, there are certain risk factors associated with its use, due to which there are certain warning labels associated with it. The injections of methylene blue in combination with serotonergic drugs can lead to the serious serotonergic syndrome. Large doses of methylene blue can lead to vomiting, nausea, abdominal pain, dizziness, precordial pain, profuse sweating, headache, hypertension, dyspnea, urinary tract irritation, and mental confusion. It may also lead to the formation of methemoglobin ultimately causing cyanosis. Young infants and patients that have a deficiency of glucose-6-phosphate dehydrogenase can cause hemolytic anemia and hemolysis. Hypersensitivity reactions including generalized urticaria, anaphylaxis, tachycardia, hypotension, and bronchospasm have been reported at the site of injection.

What are the Trade Names of Methylene Blue?

Methylene blue is traded in the market with a variety of names like desmoid Piller, Pantone, desmoid pillen, vitableu, and urolene blue. Methylene blue has many synonym names as well like methylthionine chloride, tetra methylthionine chloride, methylthioninium chloride, Aizen methylene blue, and swiss blue.

Fun Facts

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Top 41 How To Prepare Methylene Blue Indicator The 59 Latest Answer

How do you make methylene blue?

How do you make an indicator?

What is the indicator of methylene blue?

How do you dissolve methylene blue?

Is methylene blue basic or acidic?

How do you prepare methyl indicator?

What is the pH range of methyl red?

Is methylene blue a pH indicator?

How do you stain methylene blue?

Should I dilute methylene blue?

Is methylene blue stable in water?

Does ethanol dissolve methylene blue?

What is methylene blue made from?

How do you make methylene blue solution for fish?

What is methylene blue used for?

Is methylene blue poisonous?

Preparation of Indicator Solutions : Pharmaguideline

What is the Purpose of Methylene Blue?

how to prepare methylene blue indicator

Chrominfo: Preparation of methylene blue indicator solution

how to prepare methylene blue indicator

Methylene Blue Indicator 0.1% Solution

Methylene Blue – Structure, Formula, Preparation, Properties and Uses

PreparationMixedIndicator

Methylene blue staining

how to prepare methylene blue indicator

US4212971A – Process for preparing methylene blue
– Google Patents

What is the Purpose of Methylene Blue?

Methylene Blue Indicator 0.1% Solution

Methylene Blue

Leave a Comment Cancel reply

How do you make methylene blue?

How do you make an indicator?

What is the indicator of methylene blue?

How do you dissolve methylene blue?

Is methylene blue basic or acidic?

How do you prepare methyl indicator?

What is the pH range of methyl red?

Is methylene blue a pH indicator?

How do you stain methylene blue?

Should I dilute methylene blue?

Is methylene blue stable in water?

Does ethanol dissolve methylene blue?

What is methylene blue made from?

How do you make methylene blue solution for fish?

What is methylene blue used for?

Is methylene blue poisonous?

Preparation of Indicator Solutions : Pharmaguideline

What is the Purpose of Methylene Blue?

how to prepare methylene blue indicator

Chrominfo: Preparation of methylene blue indicator solution

how to prepare methylene blue indicator

Methylene Blue Indicator 0.1% Solution

Methylene Blue – Structure, Formula, Preparation, Properties and Uses

PreparationMixedIndicator

Methylene blue staining

how to prepare methylene blue indicator

US4212971A – Process for preparing methylene blue – Google Patents

What is the Purpose of Methylene Blue?

Methylene Blue Indicator 0.1% Solution

Methylene Blue

Leave a Comment Cancel reply

US4212971A – Process for preparing methylene blue
– Google Patents