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| Duetta | |
|---|---|
| Acquisition Modes | Absorbance and Fluorescence Spectrometer |
| Fluorescence Sensitivity | Water Raman SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits |
| Spectral Acquisition Rate | 510,000 nm/min |
| EEM Acquisition Rate | As fast as 1 second (sample and wavelength dependent) |
duetta fluorescence and absorbance spectrometer 주제에 대한 동영상 보기
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d여기에서 Duetta – the two-in-one Fluorescence and Absorbance Spectrometer from HORIBA – duetta fluorescence and absorbance spectrometer 주제에 대한 세부정보를 참조하세요
A Superior Spectrometer Concept – The Future of Fluorescence!
Two-in-one Fluorescence and Absorbance Spectrometer
– UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm
– Full 3-D Fluorescence EEM Acquisition in Less Than One Second
– Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman
– Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)
– High Fidelity Molecular Fingerprinting with Unique A-TEEM™(Absorbance– Transmittance Excitation Emission Matrix) Technology
– Millisecond CCD Detection of Entire Fluorescence Spectrum
Fluorescence and Absorbance in one Spectrometer!
Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.
Fluorescence in the Blink of an Eye!
Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in the world, and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.
Highest Fluorescence Sensitivity in its Class
Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations of a sample, and provides better quality data.
EzSpec™ Touch-Screen Software!
Duetta debuts HORIBA’s new generation software interface with touch-screen utility, and a selection of Apps for routine analysis.
For more information for UK and Ireland customers – contact us at [email protected]
For more information for customers outside the UK and Ireland – contact our Global Sales Team at [email protected]
duetta fluorescence and absorbance spectrometer 주제에 대한 자세한 내용은 여기를 참조하세요.
HORIBA Launches Duetta Fluorescence and Absorbance …
A two-in-one fluorescence and absorbance spectrometer*1,*2, DuettaTM achieves efficient analysis and measurement of substances, which has been …
Source: www.horiba.com
Date Published: 10/6/2021
View: 6257
Duetta™ – Fluorescence and Absorbance Spectrometer
Two-in-One Fluorescence and Absorbance Measurements. This groundbreaking spectrometer allows the measurement of both absorbance and fluorescence, and can also …
Source: www.news-medical.net
Date Published: 10/5/2021
View: 1270
Duetta™ Fluorescence and Absorbance Spectrometer from …
Get simultaneous Fluorescence and Absorbance in ONE Benchtop instrument. Features. UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm …
Source: www.labcompare.com
Date Published: 6/2/2021
View: 236
Benefits of a two-in-one fluorescence and absorbance …
Benefits of a two-in-one fluorescence and absorbance spectrometer … The HORIBA Duetta offers simultaneous fluorescence and absorbance …
Source: laboratorytalk.com
Date Published: 2/7/2021
View: 7076
New Equipment in Our Laboratory: The Duetta Fluorescence …
The Duetta Fluorescence and Absorbance Spectrometer (HORIBA) has joined the Eisele Group’s Wet Chemical Laboratory. The Duetta is capable of …
Source: eiselegroup.com
Date Published: 12/6/2022
View: 1912
주제와 관련된 이미지 duetta fluorescence and absorbance spectrometer
주제와 관련된 더 많은 사진을 참조하십시오 Duetta – the two-in-one Fluorescence and Absorbance Spectrometer from HORIBA. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 duetta fluorescence and absorbance spectrometer
- Author: HORIBA UK
- Views: 조회수 827회
- Likes: 좋아요 6개
- Date Published: 2020. 10. 9.
- Video Url link: https://www.youtube.com/watch?v=QbJdX8hu4Uw
Duetta
A Superior Spectrometer Concept
The Future of Fluorescence
Two-in-one Fluorescence and Absorbance Spectrometer
UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm
Full 3-D Fluorescence EEM Acquisition in Less Than One Second
Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman
Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)
High Fidelity Molecular Fingerprinting with Unique A-TEEM™(Absorbance-Transmittance Excitation Emission Matrix) Technology
Millisecond CCD Detection of Entire Fluorescence Spectrum
Fluorescence and Absorbance in one Spectrometer!
Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.
Fluorescence in the Blink of an Eye!
Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in the world, and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.
Highest Fluorescence Sensitivity in its Class
Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations of a sample, and provides better quality data.
EzSpec™ Touch-Screen Software!
Duetta debuts HORIBA’s new generation software interface with touch-screen utility, and a selection of Apps for routine analysis.
Watch how EzSpec software performs the Pass/Fail EzSpec Software obtains a concentration curve
EzSpec Software executes a Protein A280 concentration EzSpec Software discovers Fluorescence profiles in about 60s
A New Spectrometer Design
Fluorescence and Absorbance
Duetta’s two specialized sample detectors collect fluorescence at right angles to the excitation light, and absorbance straight through the sample.
Absolute Fluorescence Spectra with Real Time Inner Filter Effect (IFE) Correction
IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence. Duetta’s unique design applies real time IFE correction to obtain absolute fluorescence spectra at higher sample concentrations.
CCDs Collect the Entire Emission Spectrum
Ergonomic, Hands Free, Sample Access
Duetta’s CCD detection takes a snapshot of the entire fluorescence emission spectrum from 250 to 1,100 nm. (So not only is it much faster but it also gives you much more information than the competition.)
Extended NIR Wavelength Range up to 1,100 nm
Near Infrared applications are growing rapidly, and Duetta is ready to meet the challenge with fluorescence detection from 250 to 1,100 nm, well beyond the reach of standard PMTs.
Emission from Neodymium Glass
NIR detection comparing Duetta with PMT.
Easy Lamp Cartridge Replacement Lamp
Lamp cartridges are pre-aligned at the factory, guaranteeing perfect alignment upon snap-in replacement. No tools or service are required.
Smart Sample Accessory Trays
Switching from one type of sample holder to another could not be easier.
Without any tools or cables, Duetta’s spill-proof sample accessory trays are identified by EzSpec software, enable electrical power for the appropriate module (e.g. 4-position Peltier holder), and read an enabled
sensor (e.g. temperature), if available.
Fully Automated Order Sorting Filters
Duetta includes excitation and emission cut off filters for automated elimination of second order grating effects, which otherwise interfere with the fluorescence signal of interest.
EEMs Acquired in Seconds
Excitation Emission Matrix (EEM) acquired in 1 second, shows no second order scattering.
EzSpec Software
Apps at your Fingertips
From the EzSpec launch screen, each Apps icon intuitively helps you see and select the analysis you want to perform with a simple point and touch.
Apps:
Autonomous Acquisition
Pass/Fail
Build/Use a Concentration Curve
Protein A280
EzSpec lets you see your recent method and data, and your personal favorites.
Widest Range of Spectroscopic Acquisition Modes:
Fluorescence emission spectra plus Abs & IFE*
Fluorescence excitation spectra plus Abs & IFE*
Capture fluorescence value plus Abs & IFE*
Fluorescence EEM plus Abs & IFE*
Fluorescence kinetics ( emission spectra* or single point intensities)
or single point intensities) Absorbance and % Transmittance (spectra or kinetics)
* Unique to Duetta
Acquire Menu
PDF Report Generator or Export:
Includes data and results of fitting/processing
Full method details
2D and 3D graphs
Instrument and user identification information
Customizable logo
Optional and customizable signature fields
Full data and method traceability
Accessories
Optional Sample Holder Trays
SampleSnap-WJ: Thermostated and Micro-Stirred Cuvette Sample Tray
Self-identifying thermostatable, water jacketed cuvette holder (1 cm) with magnetic microstir motor. Sample tray can be installed in seconds without the need of a tool, and provides power for micro-stirrer. Requires, but does not include, external water bath.
SampleSnap-1Pelt: Single Cuvette Peltier Holder Sample Tray
Self-identifying single cuvette Peltier holder (1 cm) for automated temperature control and ramping from -15 to +105 degrees Celsius, with magnetic micro-stirrer. Sample tray can be installed in seconds without the need of a tool, and provides power and control of the Peltier device, and reads temperature. Includes external water circulator for heat sink.
SampleSnap-4Pelt: Four Position Peltier Cuvette Sample Tray
Self-identifying four position Peltier holder (1 cm) for automated temperature control and ramping from -15 to +110 degrees Celsius, with magnetic micro-stirrers under all four positions. Sample tray can be installed in seconds without the need of a tool, and provides power and control of the Peltier device, and reads temperature. Includes external water circulator for heat sink.
SampleSnap-UNI: Universal Variable Angle Sample Tray
Self-identifying sample holder with 360 degree variable angle goniometer for empirical determination of optimum sample angle. Sample tray can be installed in seconds without need of a tool. Requires, but does not include, solid sample holder accessory (SampleSnap-SS), powder sample holder accessory (SampleSnap-PWD) or cuvette holder accessory (SampleSnap-VACVT).
Sample Accessories for SampleSnap-UNI Universal Variable Angle Sample Tray
SampleSnap-SS: Solid Sample Accessory
Solid sample accessory for universal sample holder to be used with flat or solid samples such as thin films, cover slips, paper, gemstones and more. Requires, but does not include, SampleSnap-UNI.
Solid sample accessory for universal sample holder to be used with flat or solid samples such as thin films, cover slips, paper, gemstones and more. Requires, but does not include, SampleSnap-UNI. SampleSnap-PWD: Powder Sample Accessory
Powder sample accessory for universal sample holder. Requires, but does not include, SampleSnap-UNI.
Powder sample accessory for universal sample holder. Requires, but does not include, SampleSnap-UNI. SampleSnap-VACVT: Variable Angle Cuvette Sample Accessory
Cuvette sample accessory for universal sample holder. Requires, but does not include, SampleSnap-UNI.
Additional Accessories
K-210: Six pack of windows for powdered sample holder
K-151: 10 mm quartz cuvette
LampSnap: Replacement Duetta light source assembly with pre-aligned xenon arc lamp
K-AFILTER: Replacement Duetta air intake filter
* Duetta includes a standard room temperature single cuvette sample holder tray.
Applications
Duetta supports applications in a wide variety of fields
Chemistry
Life Sciences
Materials Research
Food Science
Earth Sciences
Chemistry/Photochemistry
Pyrene in a solution of A-B-A triblock copolymer micelles is solvated in the hydrophobic core solvent environment. 3D EEMs can be shown in contour, waterfall plot, or 2D overlay mode.
Biology and Biochemistry
BSA protein measured from 5° C to 60° C and the peak intensity and peak wavelength are plotted vs. temperature of the protein solution. Measured using the SampleSnap-4Pelt 4-position Peltier temperature-controlled cuvette holder.
Materials
Polymer Aggregation and Structure. Temperature dependent spectra showing polymer micellization.
Molecular Binding (FRET)
A. Kinetic spectral scans of native BSA protein with rapidly added ANS (3×10-6 M). Spectra taken every 100 ms after ANS addition. As ANS binds to native BSA, the BSA Trp emission decreases and the ANS emission increases as a result of FRET due to proximity of excited Trp and ANS.
B. Kinetic spectral scans of SDS-denatured BSA after addition of ANS. Much higher ANS concentration (4×10-5 M) is required to affect binding to enatured BSA; no FRET is observed due to increased distances between ANS and Trp.
Rare Earths
Emission spectra of a series of glass materials containing different lanthanides. Measured using the SampleSnap-Uni solid sample holder.
Protein Biophysics
Comparison of bovine serum albumin protein and the same protein denatured by sodium dodecylsulfate (SDS). The emission peak narrows and shifts 15 nm to the blue.
Pharmaceuticals
Quenching: Fluorescence monitoring of the titration of 1 M potassium iodide (KI) into a solution of 2-aminopyridine, a common small molecule drug pre-cursor.
HORIBA Launches Duetta Fluorescence and Absorbance Spectrometer
– Among the Best in the Industry for its Lightweight and Compact Footprint –
HORIBA, a global leader in fluorescence instrumentation and solutions, has announced the release of DuettaTM fluorescence and absorbance spectrometer (hereinafter, “DuettaTM”), which performs non-destructive, non-contact analysis of quantities and properties of object substances.
A two-in-one fluorescence and absorbance spectrometer*1,*2, DuettaTM achieves efficient analysis and measurement of substances, which has been hard to do with fluorescence spectrometers alone. In response to demand from customers who wish to use a fluorescence spectrometer in the limited space of a small laboratory, DuettaTM also achieves a lightweight and compact footprint that is among the best in the industry.
DuettaTM can be used to analyze a wide range of materials across multiple fields, including pharmaceutical analysis as well as analysis using fluorescence probes*3 and fluorescence dyes (bio-life science), solar batteries and LED materials (industrial materials), and minerals and rare earth metals (scarce resources and materials).
*1 Fluorescence spectrometer: A fluorescence spectrometer is an instrument that analyzes the presence, concentration, emission wavelength, and other properties of object substances by irradiating a substance with the light of a specific wavelength and capturing the light of a wavelength different from that of irradiated light (fluorescence and phosphorescence).
*2 Absorbance spectrometer: An absorbance spectrometer is an instrument that determines the amount of light absorbed by a sample (absorbance) by measuring how much light has been transmitted through a sample when irradiating a liquid sample with the light of a specific wavelength.
*3 Fluorescence probe: A fluorescence probe is a molecule that emits an intense light when bound with certain molecules. Among its uses is the ability to selectively cause cancer cells to emit light and to observe live body tissue.
DuettaTM is a fluorescence spectrometer that embodies technological knowhow gained through development of high-end spectrometers with the addition of enhanced ease-of-use features. A fluorescence spectrometer is used in a variety of fields, including in the life sciences, electronics, chemistry, agricultural science, and environmental studies. One application that is experiencing growing demand is real-time observation (e.g., live measurement of changes in body tissues and proteins using fluorescence probes).
Previously, a bottleneck existed when using fluorescence spectrometers to analyze high-concentration samples; there was a need to correct data by also using absorbance measured by an absorbance spectrometer, which added to the time before optimal data was gained. As a solution to this, the functions of an absorbance spectrometer have been added to a fluorescence spectrometer so that data can be automatically corrected to achieve efficient analysis and measurement.
1. Two-in-one solution for accurate and high-speed measurement
DuettaTM is a spectrometer that combines the functions of fluorescence and absorbance spectrometers. This two-in-one spectrometer not only broadens its field of applications but also enables the collection of accurate fluorescence spectra*4 by correcting for the inner filter effect*5 (IFE), thus allowing for better analysis of substances.
Furthermore, the built-in CCD detector extends the wavelength range for high-speed measurement.
2. Among the best in its class for its lightweight and compact footprint
DuettaTM requires 2/3 of the footprint and 1/2 the weight of competing fluorescence spectrometers. Considerable downsizing and weight reduction were realized.
(Dimensions [W x D x H]: 43.18 cm x 51.816 cm x 36.576 cm; Weight: 20.4 kg)
3. Enhanced user-friendliness
Conventional fluorescence spectrometers require specialist maintenance technicians as even the slightest misalignment inside the equipment could affect measurements. By using a consumable cartridge lamp, which customers may replace with ease, DuettaTM offers easy maintenance.
In addition, the EzSpecTM software interface with touch-screen utility (first in the industry) ensures simple measuring operation.
*4 Fluorescence spectra: Fluorescence intensity as measured by fixing the wavelength of light that object substances are irradiated with (exciting light).
*5 Inner filter effect: The inner filter effect is a phenomenon where emitted light is absorbed by a sample itself, significantly impacting measurement of high-concentration samples, thus impairing reproducibility of quantitative measurement.
Duetta™ – Fluorescence and Absorbance Spectrometer
The Duetta™ is the latest bench-top instrument from Horiba Scientific.
Two-in-One Fluorescence and Absorbance Measurements
This groundbreaking spectrometer allows the measurement of both absorbance and fluorescence, and can also be used either merely as a fluorometer, or as a UV-Vis-NIR spectrometer for absorbance measurement.
The Duetta is designed with an automatic real-time Inner Filter Effect (IFE) correction for both Primary and Secondary IFE, which is particularly important for getting a true molecular fingerprint with simultaneous measurement.
A new fingerprinting method, called A-TEEM, integrates Absorbance-Transmission and EEM (excitation-emission matrix).
Fast and Accurate
The Duetta is fitted with a high-tech Charge Coupled Device (CCD Detector) rather than traditionally used photomultiplier tubes (PMT) for fluorescence detection.
This technology allows detection of a full fluorescence spectrum in just a millisecond. A complete 3D fluorescence EEM can be attained within a second.
The CCD detector also covers NIR wavelength detection to 1100 nm.
The complete wavelength range of the Duetta is from 250 nm to 1100 nm for UV-Vis-NIR Fluorescence detection.
The maximum level of fluorescence sensitivity allows correct measurement even for low concentration samples.
Touch-Screen Software EzSpecTM
Another Horiba Scientific innovation has been embedded in the Duetta – The touch-screen software interface having a selection of Apps for routine analysis.
Duetta Highlights
Detection range from 250 nm to 1100 nm
Simultaneous measurement of fluorescence and absorbance
Rapid detection with Charge Coupled Device: whole fluorescence spectrum in just a millisecond
Real-time Inner Filter Effect correction for both Primary and Secondary IFE
A-TEEM fingerprinting integrates Absorbance-Transmission and EEM
EzSpecTM touch-screen software interface
Specifications
Duetta Duetta-Bio Acquisition Modes Simultaneous Absorbance and Fluorescence Spectrometer Fluorescence Sensitivity SNR >6,000:1 RMS, 350 nm excitation, 5 nm slits Spectral Acquisition Rate 510,000 nm/min EEM Acquisition Rate As fast as 1 second (sample and wavelength dependent) A-TEEM Acquisition Rate As fast as 30 seconds (sample and wavelength dependent) Fluorescence Detector CCD / Spectrograph Fluorescence Detector Range 250-1,100 nm Fluorescence Bandwidth 1, 2, 3, 5,10, 20 nm (excitation and emission) Absorbance Detector Silicon Photodiode Absorbance Detector Range 250-1,000 nm Absorbance Bandwidth 2, 3, 5, 10 nm Light Source 75 W Xenon arc lamp. Dedicated cartridge for snap-in replacement Excitation/Absorbance Wavelength Range 250-1,000 nm Absorbance Accuracy +/- 0.02 A Wavelength Accuracy +/- 1 nm Absorbance Range 0-2 A Computer Requirements Windows® 10/8/7 Laptop or PC with 1 USB Software EzSpec Dimensions (W x D x H) 17 x 20.4 x 14.4 inches; 43.18 x 51.816 x 36.576 cm Weight 45 pounds; 20.4 kg Polarizers (Time of Purchase) Not Available Yes (280 to 750 nm)
Benefits of a two-in-one fluorescence and absorbance spectrometer
Added: 9 Sep 2020
HORIBA used its new its two-in-one absorbable and fluorescence spectrometer, Duetta, to demonstrate…
New Equipment in Our Laboratory: The Duetta Fluorescence and Absorbance Spectrometer! — Eisele Group
The Duetta Fluorescence and Absorbance Spectrometer (HORIBA) has joined the Eisele Group’s Wet Chemical Laboratory. The Duetta is capable of detecting a wavelength range from 250 to 1,100 nm, acquiring fast Fluorescence Excitation Emission Matrix (EEM), and comes equipped with a state-of-the-art CCD camera. Being a fluorometer and a UV-Vis-NIR spectrometer in one instrument, the Duetta is able to acquire the steady-state emission and absorption spectra of our nanomaterial samples in quick succession. Let’s get some data!
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주제에 대한 기사를 시청해 주셔서 감사합니다 Duetta – the two-in-one Fluorescence and Absorbance Spectrometer from HORIBA | duetta fluorescence and absorbance spectrometer, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.
