Imark Microplate Reader | Changing The Measurement Wavelength In Bio Rad Imark Microplate Reader. 상위 292개 베스트 답변

당신은 주제를 찾고 있습니까 “imark microplate reader – Changing the measurement wavelength in Bio Rad iMark microplate reader.“? 다음 카테고리의 웹사이트 https://chewathai27.com/you 에서 귀하의 모든 질문에 답변해 드립니다: https://chewathai27.com/you/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 SANJIB KUMAR SARDAR 이(가) 작성한 기사에는 조회수 974회 및 좋아요 15개 개의 좋아요가 있습니다.

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iMark Microplate Absorbance Reader #1681130 – SelectScience

iMark™ Microplate Absorbance Reader by Bio-Rad · Automatic control of detection, data formats, and analyses · Data storage and reports for most complex …

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Date Published: 11/15/2022

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Máy đọc đĩa ELISA – iMark™ Microplate Absorbance Reader

Máy đọc đĩa ELISA – iMark™ Microplate Absorbance Reader · Các đĩa microplate chuẩn 96 giếng, 128 mm x 88 mm · Chiều cao không vượt quá 16 mm · Dạng đáy phẳng, …

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Bio-Rad® iMark™ Microplate Absorbance Reader

The reader comes with Microplate Manager® software for powerful, versatile colorimetric and turbimetric analyses as well as report analysis for raw data, …

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Bio-Rad iMark Microplate Reader – LabMakelaar Benelux

The iMark Microplate Absorbance Reader, with a wavelength range of 400 – 750 nm, is an economical, high-performance solution for a we range of …

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Bio-Rad Laboratories, Inc. iMark For Sale | Labx.com

The iMark microplate absorbance reader offers fast microplate reading speeds, extensive onboard software capabilities, a preprogrammed valation protocol (for …

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Bio-Rad iMark 750 nm Microplate Absorbance Reader

Fast microplate reading speeds · Extensive onboard assay configuration · Powerful data analysis capabilities · Convenient built-in shaker · Space-saving, we- …

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주제와 관련된 이미지 imark microplate reader

주제와 관련된 더 많은 사진을 참조하십시오 Changing the measurement wavelength in Bio Rad iMark microplate reader.. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.

Changing the measurement wavelength in Bio Rad iMark microplate reader.
Changing the measurement wavelength in Bio Rad iMark microplate reader.

주제에 대한 기사 평가 imark microplate reader

  • Author: SANJIB KUMAR SARDAR
  • Views: 조회수 974회
  • Likes: 좋아요 15개
  • Date Published: 2021. 1. 12.
  • Video Url link: https://www.youtube.com/watch?v=y48BlrohYvk

What is a microplate reader used for?

As many of you know, microplate readers are instruments used to detect biological, chemical, biochemical or physical events from samples in a microplate. This is where it all starts for your basic research, assay development and therapeutic research.

What is a microplate absorbance reader?

An absorbance microplate reader, (longform: absorbance microplate reader; shortform: absorbance reader), is a piece of equipment capable of detecting and quantifying the light photons absorbed/transmitted by a liquid sample present in a microplate, when exposed to light at a specific wavelength.

What is ELISA microplate reader?

An ELISA reader measures and quantitates the color differences in the 12 wells of the plate. ELISA readers or micro plate readers do spectrophotometry; they emit light at one wave length, and measure the amount of light absorbed and reflected by an object such as a protein.

Is microplate reader same as spectrophotometer?

A microplate reader is essentially a spectrophotometer but with a few key differences. While a spectrophotometer works with a monochromator or filter-based optics to measure events in samples just like a microtiter plate reader, a spectrophotometer works with a single small sample size.

Is a microplate reader a spectrophotometer?

Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. Monochromator- or filter-based wavelength selection. Visible only or UV/Vis spectrum, fluorescence, and luminescence.

What is UV Vis microplate reader?

Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements.

What is the use of ELISA reader?

An ELISA reader is an instrument that is used to read the fluorescent, chemiluminiscent, or chromogenic response of the ELISA in a 96-well plate. The instrument is designed to fit standard 96-well plates, and will provide quantitative information about the extent of the response for each individual well of the plate.

What are 96-well plates used for?

96-well plates are designed for High Throughput Screening (HTS), sample storage, cell culture, and DNA extraction involving a large sample size. This product has strong durability, with high temperature and chemical resistance.

What is microplate washer?

Microplate washers are laboratory instruments designed to control the procedure of washing experimental samples arranged in plate-based formats. Users load a plate and select a program; microplate washers then dispense, soak and aspirate liquids from the plate in seconds.

How does a plate reader measure absorbance?

The sample is typically placed in a cuvette through which light is sent horizontally. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each well vertically.

Microplate Readers

As many of you know, microplate readers are instruments used to detect biological, chemical, biochemical or physical events from samples in a microplate. This is where it all starts for your basic research, assay development and therapeutic research. A powerful and reliable plate reader is what you need for your scientific breakthroughs or delivering new drugs and therapeutic treatments to market. It is a crucial component and workhorse for every lab to identify the ideal drug compound to advance it from discovery to development to manufacturing.

Industries that can benefit:

For academia – the need for all major detection technologies to run everyday biochemical and cell-based assays

For pharmaceutical, biotechnology and clinical research organization – the need for high sensitivity, speed, and throughput to perform complex assays with the desire to customize for automation

Configurable Multimode Plate Readers to Future Proof Your Lab

Multimode plate readers have become very popular. Market demand has driven instrument manufacturers to progress from producing single-mode plate readers to those with multiple modes of detection. They offer flexibility and savings in cost and precious lab space, together with the convenience of employing only one instrument versus having to purchase multiple dedicated instruments.

Our multimode microplate readers offer all major detections such as Absorbance, Alpha, Luminescence, Fluorescence Intensity, Fluorescence Polarization, and Time-Resolved Fluorescence, all in one instrument. In addition to the detection technologies, our EnSight multimode plate reader also offers fast well-imaging so you can perform target-based and phenotypic screening to gain valuable insights from different perspectives. All of our plate readers are configurable so you can add technologies when you need to future proof your lab. They are ideal for applications such as cell viability/proliferation/toxicity, drug screening, pathway analysis, receptor panning, biomarkers, protein:protein interaction, gene expression, food monitoring, and epigenetics.

PerkinElmer’s portfolio of plate readers is equipped with the latest detection technologies to meet the diverse assay requirements of labs today. With over 30 years of experience in instrument development, you can expect performance and reliability from any of our microplate readers.

Software Designed for 21 CFR Part 11 Compliance

All our plate readers offer the Enhanced Security software option. It is your technological tool to help you reach compliance for 21 CFR Part 11 with features such as user management and more. Watch our short video to learn more about the Enhanced Security software option for the VICTOR® Nivo™ multimode plate reader.

Types of Detection Technologies

Absorbance Microplate Readers

If you are on the market for a new absorbance microplate reader, there are a few factors to take into account, as the performance of the instrument can significantly affect the quality of your research data.

Wavelength selection

A wavelength selection capability is mandatory for an absorbance microplate reader and is dependent on the type of detectors that are used.

Two different types of detectors are commonly used: PhotoMultiplier Tubes (PMTs) or Charge Coupled Device (CCD) spectrometers.

The PMT-based microplate reader requires a wavelength selection tool between light source and sample. Optical filters or monochromators select the desired wavelength before the light is sent to the sample. This way, only the light at a desired wavelength is transmitted through the sample, reaches the PMT, and is quantified.

To scan spectra or a range of wavelengths, the PMT-based microplate reader requires a monochromator. This selects the desired wavelengths and measures them sequentially upon mechanical movement. For measurements of multiple discrete wavelengths, multiple single measurements with different filters or a monochromator scan are required.

The CCD-spectrometer based microplate reader does not require a wavelength selection tool. The spectrometer separates the different wavelengths present in the light transmitted through the sample as these are directed onto the CCD detector. This captures the intensity of all wavelengths at once. This way, spectra, single, or multiple discrete wavelengths can be acquired in a short amount of time.

If your application needs spectral acquisition, CCD spectrometers are significantly faster than monochromators, as mechanical spectral scanning is not required.

BMG LABTECH instruments are equipped with a UV/vis spectrometer for absorbance measurements. This captures a full range spectrum from 220 to 1000 nm in less than a second/well.

Compatible microplate formats

Commonly in life science applications, basic absorbance assays like ELISA are measured in 96 well microplates. However, if you have multiple samples and/or wish to save precious reagents or time, 384 and 1536 well formats can also be used. Just make sure that your absorbance microplate reader of choice is also capable of reading the microplate format you plan on using.

Shaking and incubation

When looking for an absorbance microplate reader, you should consider the nature of your research and of the assay you want to run. Generally, shaking and incubation are two important features. Incubation is critically important when running enzymatic assays at stable temperatures, e.g. 37° C. Multiple shaking options allow a proper mixing of the samples. For applications like cell growth assays in bacteria or yeast (OD 600 ), shaking is crucial for a good oxygenation and consequent fast cell replication.

Compatibility with cuvettes

Traditionally, absorbance measurements were performed in cuvettes with a normalized path length of 1 cm. In microplates, the path length changes dependent on volume and plate format. If aqueous solutions are measured, the microplate measurement can be normalized by using the water-peak path length correction. If despite this option, for your research you still occasionally require measuring samples in cuvettes, make sure that the microplate reader of choice has either an integrated cuvette port like the SPECTROstar Nano, or plate adapters for measuring cuvettes horizontally like the LVis Plate.

Software for data reduction

Generally, the software that runs the microplate reader is a very underestimated part of the package. For some readers, the software only controls the measurement process and provides a raw data output. In other cases, it can perform complex data analysis.

A comprehensive, easy-to-use and flexible software can make life easier, simplify detection and data analysis so that you can get the most out of your instrument.

The most useful calculation features include automatic blank subtraction, automatic calculation of sample concentrations based on standard curves for ELISA assays or on the Beer-Lambert law, 260/280 ratios, path length corrections, and enzymatic constants such as K m and V max .

On the BMG LABTECH software package, the most common assays have predefined quick-run assay-specific protocols and dedicated data reduction templates. Through these dedicated solutions, data acquisition and analysis are performed with a single mouse click, enabling researchers to carry out experiments quickly and easily.

iMark Microplate Absorbance Reader #1681130

As the instrument is user-friendly, we have achieved high-quality data, minimized sample volume, and achieved reproducible results. Functions and protocols for kinetics measurements of enzymatic reactions are very useful for biochemical research. Flexible and clear protocols, varying wavelength and convenient interface make routine laboratory work easier and decrease the time taken for analysis.

We have had an old model of reader from Bio-Rad for over 20 years. It has been very reliable! We bought the new model when our computer connected to the old one died. The new model is even better, it’s fast and quiet. I hope it has the same lifespan as the old one.

Excellent system to read your microplates with ease for handy protocols and great detection of molecules. I am very happy to be the part of the team which uses this instrument. I use this instrument very frequently for testing purposes and it provides me with great outputs. I highly recommend this instrument for molecular biology labs.

The iMark™ microplate absorbance reader offers reading speeds of 6 sec at single wavelength detection, onboard software capabilities including protocols for kinetic assays, a preprogrammed validation protocol, a built-in plate shaker, and a built-in thermal printer. The instrument can read flat-, U-, or V-bottom microplates or 8- or 12-well strip plates. The iMark reader can be used as a stand-alone instrument or controlled by a PC or Mac with Microplate Manager® 6 software. It is provided with six filters (415–750 nm).

Benefits

Automatic control of detection, data formats, and analyses

Data storage and reports for most complex photometric applications

Easy configuration, storage, and application of customized protocols

Proactive and prompt maintenance

Comprehensive data analysis

Accommodation of various validation protocols including installation qualification and operational qualification (IQ/OQ)

The validation protocol is for the Checkmark™ reader performance check plate.

Bio-Rad® iMark™ Microplate Absorbance Reader

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Bio-Rad iMark Microplate Reader

The iMark Microplate Absorbance Reader, with a wavelength range of 400 – 750 nm, is an economical, high-performance solution for a wide range of applications, including immunoassays with colorimetric substrates, such as ELISA, and protein assays such as Bradford and Lowry. The instrument can be used as a stand-alone reader or be controlled by a PC or Mac with Microplate Manager 6 Software (not included).

Fast microplate reading speeds

Extensive onboard assay configuration

Powerful data analysis capabilities

Convenient built-in shaker

Space-saving, wide-format internal thermal graphical printer

Simplified standalone operation

Compact, easy-to-use, and lightweight design

Compatible with different types of 96-well microplates (flat, U-, V-bottom) and 8- or 12-well strip plates; maximum plate height: 16 mm

Wavelength range: 400 – 750 nm

Photometric range: 0.0–3.5 OD

Linearity: ≤1.0% from 0.0 – 2.0 OD, ≤2.0% from 0.0 – 3.0 OD

Accuracy: ≤1.0% or 0.010 from 0.000 – 3.000 OD at 490 nm

Precision: 1.0% or 0.005 OD from 0.0 – 2.0 OD, 1.5% from 2.0 – 3.0 OD

Resolution: 0.001 OD

Filter wheel capacity: 8

Plate shaking: Low, mid, high

Duration: 0 – 999 sec

Read time: 6 sec at single wavelength, 10 sec at dual wavelengths

Dimensions (WxDxH): 346 x 377 x 164 mm

Weight: 5.5 kg

Bio-Rad Laboratories, Inc. iMark For Sale

Bio-Rad Laboratories, Inc. iMark

Find the Bio-Rad Laboratories, Inc. iMark you need and shop from a wide selection of vendors. Buy now or request a price quote.

The iMark microplate absorbance reader is an economical, high-performance solution for a wide range of photometric applications. The iMark reader represents the next generation of Bio-Rad’s popular Model 550 and Model 680 series Microplate readers. For over two decades, Bio-Rad absorbance microplate readers have hit the highest marks for reliability and efficiency in thousands of laboratories around the world.

The iMark microplate absorbance reader offers fast microplate reading speeds, extensive onboard software capabilities, a preprogrammed validation protocol (for the Checkmark reader performance check plate), and a built-in printer in a compact, lightweight unit. The instrument can be used as a stand-alone reader or be controlled by a PC or Mac with Microplate Manager 6 software.

The wavelength range of the iMark reader is 400–750 nm, permitting many applications, such as ELISAs with colorimetric substrates and protein assays (such as Bradford and Lowry).

Kinetic reading can also be programmed with onboard software or controlled by Microplate Manager software. The iMark reader’s onboard software is well suited for life science and clinical laboratories. The software includes security login features that allow locking and unlocking of key protocol settings by the administrator, generous memory capacity for protocol creation and storage, comprehensive curve-fit selections, and extensive reporting functions. Onboard software includes preprogrammed protocols for the Bio-Rad Checkmark reader performance verification kit, to validate the IQ/OQ/PQ of the iMark reader in compliance with GLP. Other life science and specialized protocols are also included, such as for the Bio-Rad TSE kits and a growing number of food testing and clinical kits.

The iMark reader comes with six standard absorbance filters: 415, 450, 490, 595, 655, and 750 nm. Bio-Rad also supplies custom filters at 5 nm intervals from 400 to 750 nm on request. A wide-format internal thermal graphical printer saves lab space and simplifies stand-alone operation.

Bio-Rad iMark 750 nm Microplate Absorbance Reader, Plate Reader, माइक्रोप्लेट रीडर – Bio-Rad Laboratories India Private Limited, Gurgaon

The iMark™ Microplate Absorbance Reader, with a wavelength range of 400–750 nm, is an economical, high-performance solution for a wide range of applications, including immunoassays with colorimetric substrates, such as ELISA, and protein assays such as Bradford and Lowry. The instrument can be used as a stand-alone reader or be controlled by a PC or Mac with Microplate Manager® 6 Software

Fast microplate reading speeds

Extensive onboard assay configuration

Powerful data analysis capabilities

Convenient built-in shaker

Space-saving, wide-format internal thermal graphical printer

Simplified standalone operation

Compact, easy-to-use, and lightweight design

Compatible with different types of 96-well microplates (flat, U-, V-bottom) and 8- or 12-well strip plates; maximum plate height: 16 mm

Wide applicability for life science and clinical laboratories

Programmable kinetic reading

Security login that allows locking and unlocking of key protocol settings by the administrator

Generous memory capacity for protocol creation and storage

Comprehensive curve-fit selections

Extensive reporting functions

Preprogrammed protocols for the Checkmark™ Reader performance verification kit, to validate the IQ/OQ/PQ of the iMark Reader in compliance with GLP

Other life science and specialized protocols included such as for the Bio-Rad TSE kits and a growing number of food testing and clinical kits

Bio-Rad also supplies custom filters at 5 nm intervals from 400 to 750 nm on request.

키워드에 대한 정보 imark microplate reader

다음은 Bing에서 imark microplate reader 주제에 대한 검색 결과입니다. 필요한 경우 더 읽을 수 있습니다.

이 기사는 인터넷의 다양한 출처에서 편집되었습니다. 이 기사가 유용했기를 바랍니다. 이 기사가 유용하다고 생각되면 공유하십시오. 매우 감사합니다!

사람들이 주제에 대해 자주 검색하는 키워드 Changing the measurement wavelength in Bio Rad iMark microplate reader.

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주제에 대한 기사를 시청해 주셔서 감사합니다 Changing the measurement wavelength in Bio Rad iMark microplate reader. | imark microplate reader, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.

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