당신은 주제를 찾고 있습니까 “anti human fab antibody – Anti Human Antibodies – Products for Serological Immunoassays“? 다음 카테고리의 웹사이트 https://chewathai27.com/you 에서 귀하의 모든 질문에 답변해 드립니다: Chewathai27.com/you/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 Jackson ImmunoResearch Laboratories 이(가) 작성한 기사에는 조회수 588회 및 좋아요 6개 개의 좋아요가 있습니다.
Anti-human IgG (Fab specific) antibody can be used in Ouchterlony double diffusion. Due to lack of interspecies cross reactivity to mouse or rat ascites fluids, this antibody is also ideal for screening human monoclonal antibodies produced by hybridoma cells grown in vivo.Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target.The Anti-Human IgG (H+L), HRP Conjugate, antibody binds to both heavy and light chains for all human IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution.
Table of Contents
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d여기에서 Anti Human Antibodies – Products for Serological Immunoassays – anti human fab antibody 주제에 대한 세부정보를 참조하세요
Anti-Human antibody selection guide for serological tests, including:
• Common serological assay types
• Antibody format
• Antibody specificity and sensitivity
• Host species considerations
• Eliminating cross-reactivity
• Conjugate options
Read more about our anti-Human antibodies here: https://www.jacksonimmuno.com/technical/products/groups/anti-human-secondary-antibodies/serology
anti human fab antibody 주제에 대한 자세한 내용은 여기를 참조하세요.
Anti-Human IgG Fab fragment antibody [4A11] (ab771) – Abcam
Mouse monoclonal Human IgG Fab fragment antibody [4A11]. Valated in WB, Flow Cyt and tested in Cow, Human. Cited in 3 publication(s).
Source: www.abcam.com
Date Published: 7/24/2021
View: 1800
Anti-Human IgG (Fab specific)−Peroxidase antibody …
Anti-Human IgG (Fab specific)−Peroxase antibody produced in goat (affinity isolated antibody); Suitable for direct ELISA; Peroxase-conjugated goat …
Source: www.sigmaaldrich.com
Date Published: 9/15/2022
View: 3706
Mouse anti-Human IgG Fab, PE (MA1-10377)
Invitrogen Anti-Human IgG Fab Secondary Antibody, Catalog # MA1-10377. Tested in Flow Cytometry (Flow) applications. Supplied as 100 µg purified secondary …
Source: www.thermofisher.com
Date Published: 8/8/2021
View: 4682
Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb
As a mouse monoclonal antibody, the unique GenScript Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb has a high specificity to the human IgG Fab portion …
Source: www.genscript.com
Date Published: 8/14/2021
View: 7017
Recombinant Mouse Anti-Human IgG Fab antibody
Recombinant Mouse Anti-Human IgG Fab antibody. Suitable for use in WB, FC, ELISA. Belongs to the family.
Source: www.creativebiolabs.net
Date Published: 5/27/2021
View: 5197
AffiniPure Fab Goat Anti-Human IgG (H+L) Secondary Antibody
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies …
Source: www.jacksonimmuno.com
Date Published: 1/13/2022
View: 3155
Anti-Human IgG Fab Antibody [4A11] (A86804)
Buy mouse monoclonal (4A11) antibody to Human IgG Fab (A86804). Applications: WB, ELISA and more. Reactivity: Human. ✔️ 100% Guarantee ✔️ FREE Shipping.
Source: www.antibodies.com
Date Published: 7/4/2022
View: 3238
주제와 관련된 이미지 anti human fab antibody
주제와 관련된 더 많은 사진을 참조하십시오 Anti Human Antibodies – Products for Serological Immunoassays. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 anti human fab antibody
- Author: Jackson ImmunoResearch Laboratories
- Views: 조회수 588회
- Likes: 좋아요 6개
- Date Published: 2021. 1. 28.
- Video Url link: https://www.youtube.com/watch?v=Ikss-fgBpqY
What is anti-human antibody?
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target.
What is anti-human IgG HRP?
The Anti-Human IgG (H+L), HRP Conjugate, antibody binds to both heavy and light chains for all human IgG subclasses. As with all antibodies, in certain applications some species-dependent antigen-dependent cross-reactivity may be observed. The product (unless otherwise noted) is supplied as a 1mg/ml solution.
What IgG fab?
Fab (50,000 daltons) is a monovalent fragment that is produced from IgG and IgM, consisting of the VH, CH1 and VL, CL regions, linked by an intramolecular disulfide bond.
Why are anti-human antibodies used?
Serological tests for human samples should use Anti-Human Secondary Antibodies to minimize non-specific binding, which can lead to high background signals and false positives. Anti-Human Secondary Antibodies are developed in a range of host species including alpaca, donkey, goat, mouse and rabbit.
What do anti-human antibodies bind to?
Anti-human antibodies bind to primary antibodies to aid in the detection, sorting, and purification of target antigens, so as not to interfere with the binding procedure of the primary antibodies with the antigens. This improves the signal for higher sensitivity and selectivity.
How do you make Fab from antibodies?
The primary method is via enzymatic/chemical cleavage of the whole antibody, in which the whole antibody is cleaved by enzyme (such as papain, pepsin, and ficin) to form F(ab’)2 fragments, followed by the reduction of those fragments to yield Fab fragments [2].
Is a Fab a protein?
Fabs consist of two polypeptide chains expressed in bacteria. One contains the polypeptide-coding region of the light chain variable domain plus the constant domain, and the other contains the heavy chain variable domain plus one constant domain.
What are 4 ways which antibodies work?
Examples of antibody functions include neutralization of infectivity, phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), and complement-mediated lysis of pathogens or of infected cells.
Anti-Human IgG (Fab specific) antibody produced in goat affinity isolated antibody, buffered aqueous solution
General description
Anti-Human IgG (Fab specific) antiserum is produced in goat using purified human IgG Fab fragment as the immunogen. Affinity isolated antibody is obtained from goat anti-human IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. IgG is a larger molecule with a molecular weight of 150 kilo daltons (kDa). IgG has two light chains (L) and two heavy chains (H). The light chain can be either lambda (λ) or kappa (κ) chain. Functionally, immunoglobulins have constant region and variable region. Light chain has one variable region in the N-terminal VL and followed by one constant domain CL. The light chain is approximately about 25 kDa. The heavy chain has one variable region in the N-terminal and followed by three constant domains. In between the first (C1H) and second constant (C2H) domain in the spacer hinge region which connects both the heavy chains. Each heavy chain is about 55 kDa.
Anti-Human IgG Fab fragment antibody [4A11] (ab771)
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Goat anti-Human Antibodies
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label … View more Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Anti-Human IgG (H+L), HRP Conjugate
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Thermo Fisher Scientific – VN
Antibodies are powerful tools for protein and molecular detection and purification. Although whole antibodies (usually IgG or IgM) are ideal for most immunoassay applications, the performances of certain procedures are enhanced by using antibody fragments, such as Fab and F(ab’)2. This article reviews the benefits, types and methods for preparing antibody fragments.
Introduction
Sometimes it is useful to study or make use of the activity of one portion of an immunoglobulin without interference from other portions of the molecule. It is possible to selectively cleave the immunoglobulin molecule into fragments that have discrete characteristics. Antibody fragmentation is accomplished using reducing agents and proteases that digest or cleave certain portions of the immunoglobulin protein structure. Although fragmentation of all immunoglobulin classes is possible, only procedures for fragmentation of mouse, rabbit, and human IgG and IgM have been well characterized. The two groups of antibody fragments of primary interest are (a) antigen-binding fragments such as Fab and (b) class-defining fragments such as Fc that do not bind antigen. Several types of antigen-binding fragments are possible, but each contains at least the variable regions of both heavy and light immunoglobulin chains (VH and VL, respectively) held together (usually by disulfide bonds) so as to preserve the antibody-binding site. Fc fragments consist of the heavy chain constant region (Fc region) of an immunoglobulin and mediate cellular effector functions. Antibody fragmentation is somewhat laborious, requires optimization of enzyme-mediated digestion of the protein and necessitates an ample supply of antibody (e.g., 10mg) to make it reasonably efficient. For these reasons, fragmentation is usually performed only when the antibody of interest is available in large quantity and the particular application demands it.
Note
Primary antibodies (1°Ab) are seldom offered commercially as ready-made fragments because there is limited demand for any given item. For this reason, except with custom antibody production, fragmentation is an activity for each individual laboratory to perform for its specific needs.
Antibody IgG structure and cleavage sites for fragmentation. Useful antibody fragments, including half-IgG, Fab, F(ab’)2 and Fc, can be produced by reduction of hinge-region disulfides or digestion with papain, pepsin or ficin proteolytic enzymes.
Advantages of antibody fragments
Because of their smaller size as functional components of the whole molecule, antibody fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications: Reduced nonspecific binding from Fc interactions (many cells have receptors that bind the Fc region)
Ability to control Fc-binding to Protein A or Protein G in experiments involving immunoprecipitation and Western blotting
More efficient penetration of tissue sections, resulting in improved staining in immunohistochemistry (IHC)
Potentially higher sensitivity in antigen detection in solid phase applications as a result of reduced steric hindrance from large protein epitopes
Elimination of Fc-associated effector functions (e.g,. complement fixation) in antigen-antibody binding studies
Simpler system for studying the structural basis for immune recognition using X-ray crystallography or NMR
Lower immunogenicity than intact antibody for experiments in vivo
Types of antibody fragments
F(ab’)2, Fab, Fab’ and Fv are antigen-binding fragments that can be generated from the variable region of IgG and IgM. These antigen-binding fragments vary in size (MW), valency and Fc content. Fc fragments are generated entirely from the heavy chain constant region of an immunoglobulin. These and several additional unique fragment structures can be generated from pentameric IgM, including an “IgG”-type fragment, an inverted “IgG”-type fragment, and a pentameric Fc fragment. The names (nomenclature) and structures of typical IgG fragments are illustrated in the following diagram and summarized below.
F(ab’)2 fragments
F(ab’)2 (110,000 daltons) fragments contain two antigen-binding regions joined at the hinge through disulfides. This fragment is void of most, but not all, of the Fc region.
Fab’ fragments
Fab’ (55,000 daltons) fragments can be formed by the reduction of F(ab’)2 fragments. The Fab’ fragment contains a free sulfhydryl group that may be alkylated or utilized in conjugation with an enzyme, toxin or other protein of interest. Fab’ is derived from F(ab’)2; therefore, it may contain a small portion of Fc.
Fab fragments
Fab (50,000 daltons) is a monovalent fragment that is produced from IgG and IgM, consisting of the VH, CH1 and VL, CL regions, linked by an intramolecular disulfide bond.
Fv fragments
Fv (25,000 daltons) is the smallest fragment produced from IgG and IgM that contains a complete antigen-binding site. Fv fragments have the same binding properties and similar three-dimensional binding characteristics as Fab. The VH and VL chains of the Fv fragments are held together by non-covalent interactions. These chains tend to dissociate upon dilution, so methods have been developed to cross-link the chains through glutaraldehyde, intermolecular disulfides or a peptide linker.
“rIgG” fragments
“rIgG” refers to reduced IgG (75,000 daltons) or half-IgG. It is the product of selectively reducing just the hinge-region disulfide bonds. Although several disulfide bonds occur in IgG, those in the hinge-region are most accessible and easiest to reduce, especially with mild reducing agents like 2-mercaptoethylamine (2-MEA). Half-IgG are frequently prepared for the purpose of targeting the exposing hinge-region sulfhydryl groups that can be targeted for conjugation, either antibody immobilization or enzyme labeling.
Fc fragments
Fc (50,000 daltons) fragments contain the CH2 and CH3 region and part of the hinge region held together by one or more disulfides and noncovalent interactions. Fc and Fc5µ fragments are produced from fragmentation of IgG and IgM, respectively. The term Fc is derived from the ability of these antibody fragments to crystallize. Fc fragments are generated entirely from the heavy chain constant region of an immunoglobulin. The Fc fragment cannot bind antigen, but it is responsible for the effector functions of antibodies, such as complement fixation.
IgG—Preparing Fab, F(ab’)2 and Fc fragments
The hinge region of an immunoglobulin monomer (IgG) is readily accessible to proteolytic attack by enzymes. Cleavage at this point produces F(ab’)2 or Fab fragments and the Fc fragment. The Fc fragment may remain intact or become further degraded, depending upon the enzyme and conditions used. Proteolytic IgG fragmentation using three different enzymes is discussed below. Traditionally, proteolysis was accomplished in solution using free enzyme. We have developed immobilized enzyme products that enable better control of digestion and efficient separation of reaction-products from the protease. Thus, the diagrams featured below refer to enzyme “resins.” Most procedures also include Protein A resin antibody purification steps to separate Fab and Fc fragments.
Papain digestion: Fab from IgG
Papain is a nonspecific, thiol-endopeptidase that has a sulfhydryl group in the active site, which must be in the reduced form for activity. When IgG molecules are incubated with papain in the presence of a reducing agent, one or more peptide bonds in the hinge region are split, producing three fragments of similar size: two Fab fragment and one Fc fragment (1). When Fc fragments are of interest, papain is the enzyme of choice because it yields an intact 50,000-dalton Fc fragment.
Antibody Fab preparation by papain digestion and fragmentation.
Papain
Thiol-type protease
MW 23,000
Isoelectric point pI = 1.5
pH optimum 6.5 (4 to 9.5)
A280 at 1% = 25 Papain is primarily used to generate Fab fragments, but it also can be used to generate F(ab’)2 fragments (2). To prepare F(ab’)2 fragments, the papain is first activated with 10mM cysteine. The excess cysteine is then removed by gel filtration. If no cysteine is present during papain digestion, F(ab’)2 fragments can be generated. These fragments are often inconsistent, and reproducibility can be a problem. If the cysteine is not completely removed, overdigestion can be a problem (2). Crystalline papain is often used for the digestion of IgG; however, it is prone to autodigestion. Mercuripapain, which is less prone to autodigestion than crystalline papain, can be used; however, both of these non-immobilized enzymes require an oxidant to terminate digestion. Immobilized papain (i.e., Papain Agarose Resin) is the preferred reagent because it allows for easy control of the digestion reaction and quick removal of enzyme from the digestion products following incubation. Thus, there is no need to develop an ion exchange method for separating the fragments from the enzyme. The use of immobilized papain alsos prevent formation of antibody-enzyme adducts, which can occur when using the soluble form of sulfhydryl proteases (such as papain). These adducts can be detrimental to fragments in the presence of reductants. Immobilization also increases stability of the enzyme against heat denaturation and autolysis and results in longer maintenance of activity. Regeneration and reuse of papain resin, which decreases costs. Cleavage can be regulated by digestion time or flow rate through a column, yielding reproducible digests. Our Pierce Fab Preparation Kits are been optimized for human IgG digestions. The kits also can be used successfully for mouse and rabbit IgG digestions, and suggestions on how to vary the protocols for other species of IgG are provided with the kit. The procedures requires that the IgG is able to be bound by Protein A, as it is used to separate Fc from Fab fragments.
Pepsin digestion: F(ab’)2 from IgG
Pepsin is a nonspecific endopeptidase that is active only at acid pH. It is irreversibly denatured at neutral or alkaline pH. Digestion by the enzyme pepsin normally produces one F(ab’)2 fragment and numerous small peptides of the Fc portion. The resulting F(ab’)2 fragment is composed of two disulfide-connected Fab units. The Fc fragment is extensively degraded, and its small fragments can be separated from F(ab’)2 by dialysis, gel filtration or ion exchange chromatography.
Antibody F(ab’)2 preparation by pepsin digestion and fragmentation.
Pepsin
Acid-type protease
MW 35,000
Isoelectric point pI = 11
pH optimum 1 (1 to 5)
A280 at 1% = 14.7 F(ab’)2 can be separated by mild reduction into two sulfhydryl-containing, univalent Fab’ fragments. The advantage of Fab’ fragments is that they can be conjugated to detectable labels directly through their sulfhydryl groups, ensuring that the active binding site remains unhindered and active. Use 2-Mercaptoethylamine•HCl (2-MEA) for mild reduction of F(ab’)2 fragments. The free sulfhydryls of each Fab’ can be targeted for conjugation, or they can be blocked with an alkylating reagent, such as N-Ethylmaleimide (NEM) to prevent re-formation of the F(ab’)2. Immobilized pepsin (Pepsin Agarose Resin) can be substituted for free pepsin in any application. Use of pepsin resin allows one to control digestion by quickly removing the enzyme from the sample to stop the reaction. This eliminates the need to develop an ion exchange method to separate the fragments from the protease. Also, immobilization increases the stability of the pepsin against heat denaturation and autolysis, resulting in longer maintenance of activity. Our Pierce F(ab’)2 Preparation Kits have been optimized for human IgG digestions. The kits also can be used successfully for rabbit and mouse IgG digestions.
Ficin digestion: Mouse IgG1 fragments
Ficin is a thiol protease that can digest mouse monoclonal IgG1 into either F(ab’)2 or Fab fragments, depending on the concentration of cysteine included. Ficin will generate F(ab’)2 in the presence of 4mM cysteine. Fab fragments result with ficin in the presence of 25mM cysteine.
Mouse IgG1 Fab and F(ab’)2 preparation by ficin digestion and fragmentation.
Ficin
Thiol-type protease
MW 26,000
Isoelectric point pI = 1.5
pH optimum 6.5 (4 to 9.5)
A280 at 1% = 21 Ficin cleavage produces F(ab’)2 fragments of nearly identical size to those obtained from IgG by pepsin but with immunoreactivities and affinities comparable to those of intact IgG1 antibody (3). By increasing the concentration of cysteine activator, Fab antigen-binding fragments can be generated (4). The integrity of the resultant antigen-binding fragments is aided by the neutral pH conditions of the ficin digestion. Ficin is the preferred protease for production of fragments of murine monoclonal IgG1. Although F(ab’)2 can be generated from mouse IgG1 using pre-activated papain (5), efficiency and reproducibility with papain are difficult to obtain (6). Immobilized ficin (Ficin Agarose Resin) enables better control of the digestion reaction than free ficin, as it results in antibody fragments that are free of autodigestion products. In addition, use of ficin resin allows complete and rapid removal of ficin from the antibody fragment products. Our Pierce IgG1 Fab and F(ab’)2 Preparation Kit was developed to allow gentle production and purification of either Fab or F(ab’)2 fragments from intact murine IgG1 antibodies. The type of fragment produced is controlled by the specific concentration of cysteine activator used during the digestion.
IgM fragmentation
The large size of IgM creates difficulties in applications in which IgM is used for in vitro experiments. For example, intact IgM does not effectively penetrate tissues for immunohistochemical studies. Thus, it is necessary to produce smaller, active fragments for these types of applications. Also, because IgM molecules have difficulty permeating cell membranes, they are not ideal for use in vivo. Fragments are cleared more rapidly than intact IgM. F(ab’)2, Fab’, Fab and Fv fragments can produced from IgM function in much the same way as these same types of fragments from IgG. However, compared to those in IgG, individual antigen-binding sites in IgM generally have lower binding affinities, which normally are compensated in the complete IgM by its pentameric form. Thus, because F(ab’)2 fragments are divalent, and they may be a superior alternative to Fab fragments for antibodies with low affinity. F(ab’)2 fragments have higher avidity than the Fab and Fab’ fragments. F(ab’)2 fragments can precipitate antigen. Fab and Fab’ are univalent molecules that cannot precipitate antigen. Fab and Fab’ fragments have a decreased binding strength, and normally stable antigen-antibody complexes may dissociate during washes in certain applications.
Methods of IgM antibody fragment preparation.
Each species of IgM reacts differently to enzymatic cleavage and reduction. For example, mouse and human IgM differ structurally in the manner in which the monomers are linked to give the pentameric form, primarily as a result of differences in the location of disulfides between the monomers (7). Also, oligosaccharide components, which may hinder enzymatic cleavage, vary among species. Therefore, optimal digestion and reduction conditions for one species may not be effective for another. Fragmentation of IgM by proteolyic enzymes proceeds differently from IgG fragmentation. These changes are related to differences in structure. The heavy (µ) chains are folded into multiple globular domains, and IgM has a carbohydrate-rich Cµ2 domain in place of the proline-rich hinge-region of IgG. Because of these features, papain produces heterogeneous fragments from IgM. Pepsin (see discussion above) can be used to produce F(ab’)2, Fab and Fv fragments from IgM. Many methods have been developed that use pepsin to produce different IgM fragments from different species (8). Trypsin is a serine protease that reacts optimally at pH 8.0. In general, increasing the enzyme:substrate ratio and/or the temperature will increase the rate of digestion. Trypsin can generate F(ab’)2, Fab, “IgG”-type and Fc5µ fragments from IgM. Fragmentation was studied using trypsin with and without urea pre-treatment (8). Urea alters the susceptibility of the domains to digestion and produces different fragments from those digested in aqueous buffer. Many other procedures have been developed to digest IgM using trypsin (9). Controlled reduction can be performed using 2-Mercaptoethylamine•HCl (2-MEA) to obtain “IgG”-type and/or reduced IgG (“rIgG”) varied proportions, depending upon reduction time and/or temperature (10). Partial reduction of mouse IgM also produces an inverted “IgG”-type fragment.
Trypsin
Serine-type protease
MW 24,000
Isoelectric point pI = 1.5
pH optimum 8
A280 at 1% = 14.3
References
Anti-Human IgG (Fab specific Peroxidase antibody) Anti Human Fab Antibody
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,. Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,. Antibody is isolated from anti-human IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fab fragment of human IgG. Anti-Human IgG is conjugated to peroxidase and then further purified to remove unconjugated material.
Anti-Human IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a-phenylene-diamine substrate solution (Sigma).
Disclaimer
Mouse anti-Human IgG Fab, PE (MA1-10377)
Target Information
Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Optimal working dilutions must be determined by the end user.
Recombinant Mouse Anti-Human IgG Fab antibody
Recombinant Mouse Anti-Human IgG Fab antibody (CAT#: MOB-0294MC)
Recombinant Mouse Antibody is capable of binding to Human IgG Fab, expressed in Chinese Hamster Ovary cells (CHO).
Specific Inquiry Size: 500 µg 1 mg 2 mg 5 mg 10 mg 50 mg 100 mg 1 g Other
Conjugation: NONE PE APC Biotin FITC HRP Oligonucleotide Azide Other
Endotoxin: Regular batch (without endotoxin removal) <1EU/mg <0.1EU/mg Other Purity: SEC-HPLC>90% SEC-HPLC>95% SEC-HPLC>98% SDS-PAGE>95% Other
Fc Engineering: Fc silenced ADCC enhanced CDC enhanced Reduced CDC Increased half-life ADCC and CDC enhanced Reduced ADCC and CDC Reduced Fab-arm exchange Other Add To Basket
Datasheet
MSDS
COA Datasheet MSDS Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Specifications
Immunogen
Human IgG Fab antibody was raised against full length native protein (purified) (Human). Host Species
Mouse Specificity
This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by Western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions. Species cross-reactivity: Cross-reacts with Human. Not yet tested in other species. Species Reactivity
Human Conjugate
Unconjugated
Target
Alternative Names
fragment antigen-binding (Fab) fragment; Fab; IgG
Product Notes
This is a product of Creative Biolabs’ Hi-Affi™ recombinant antibody portfolio, which has several benefits including: • Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
Customer Reviews and Q&As
Submit a review or a question Thare are currently no Customer reviews or questions for MOB-0294MC. Click the button above to contact us or submit your feedback about this product. View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
* Abbreviations
AffiniPure Fab Goat Anti-Human IgG (H+L) Secondary Antibody
Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6None
20-40 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Anti-Human IgG Fab Antibody [4A11] (A86804)
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
키워드에 대한 정보 anti human fab antibody
다음은 Bing에서 anti human fab antibody 주제에 대한 검색 결과입니다. 필요한 경우 더 읽을 수 있습니다.
이 기사는 인터넷의 다양한 출처에서 편집되었습니다. 이 기사가 유용했기를 바랍니다. 이 기사가 유용하다고 생각되면 공유하십시오. 매우 감사합니다!
사람들이 주제에 대해 자주 검색하는 키워드 Anti Human Antibodies – Products for Serological Immunoassays
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주제에 대한 기사를 시청해 주셔서 감사합니다 Anti Human Antibodies – Products for Serological Immunoassays | anti human fab antibody, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.
