당신은 주제를 찾고 있습니까 “bead beater homogenizer – Bead Genie – Laboratory Bead Beater“? 다음 카테고리의 웹사이트 https://chewathai27.com/you 에서 귀하의 모든 질문에 답변해 드립니다: https://chewathai27.com/you/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 ScientificIndustries 이(가) 작성한 기사에는 조회수 61,510회 및 440703 Like 개의 좋아요가 있습니다.
bead beater homogenizer 주제에 대한 동영상 보기
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d여기에서 Bead Genie – Laboratory Bead Beater – bead beater homogenizer 주제에 대한 세부정보를 참조하세요
Bead beater ideal for lysing, extraction, or homogenization applications, utilizing an aggressive vertical mixing motion. Supplied with holders to hold vertically 12 microtubes (1.5 or 2.0ml), 6 15ml tubes, or 3 50ml tubes, and 12 microtubes (1.5 or 2.0ml) horizontally. Beads are also available and sold separately for a complete bead beating solution. The Bead Genie, built in the USA, is compact compared to most bead beaters and homogenizers, yet heavy and durable.
bead beater homogenizer 주제에 대한 자세한 내용은 여기를 참조하세요.
Bead Beater Homogenizers | MP Biomedicals
The FastPrep® family of benchtop instruments utilize bead-beating technology to lyse, homogenize and grind routine and difficult samples in 40 seconds or less.
Source: www.mpbio.com
Date Published: 10/7/2021
View: 9597
Bead Mill Homogenizers
Bead mill homogenizers use beads to homogenize – no surprises there. The beads are vigorously shaken to break up tissue and disrupt cells.
Source: homogenizers.net
Date Published: 9/1/2022
View: 983
Bead beater cell lysis compared to rotor stator homogenizers
Bead mill homogenizing is self-contained in a custom bead mill tube…however, this means you are either restricted to the type of tubes you can continue working …
Source: proscientific.com
Date Published: 11/18/2021
View: 5418
BeadBeater – BioSpec Products
Monitoring cell lysis. The BeadBeater will disrupt over 90% of the cells in about 2-5 minutes of operation. The homogenization procedure involves cell …
Source: www.biospec.com
Date Published: 7/15/2021
View: 4292
SpeedMill PLUS Bead Mill Homogenizer – tissue lyser
Effective bead mill homogenization for many sample types … The SpeedMill PLUS is a highly efficient beadmill homogenization system for various starting …
Source: www.westburg.eu
Date Published: 4/24/2021
View: 7101
BEAD BEATER – MRC Lab
Bead Beater by MRC. … substantial sample warming that occurs with other bead beating homogenizers. … Digital Benchtop Homogenizer for tissues and cells.
Source: www.mrclab.com
Date Published: 8/10/2022
View: 1232
주제와 관련된 이미지 bead beater homogenizer
주제와 관련된 더 많은 사진을 참조하십시오 Bead Genie – Laboratory Bead Beater. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 bead beater homogenizer
- Author: ScientificIndustries
- Views: 조회수 61,510회
- Likes: 440703 Like
- Date Published: 2021. 3. 15.
- Video Url link: https://www.youtube.com/watch?v=-ELJfCWEWdE
Bead Mill Homogenizers
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Bead Mill Homogenizers
Bead Mill Homogenizers
Bead mill homogenizers use beads to homogenize – no surprises there. The beads are vigorously shaken to break up tissue and disrupt cells. Bead mills are great for breaking up tissue and many are high-throughput. Furthermore, because there are no probes, samples are self-contained and there is a reduced opportunity for contamination (both cross-contamination as well as contamination of the laboratory environment). You can learn more about bead mill homogenization in the Application Center.
Each different brand of bead mill has a different mechanism for shaking the beads, each with their pros and cons. Click on an instrument below for more information, and don’t hesitate to ask us a question if you have any.
Bead beater cell lysis compared to rotor stator homogenizers
False
Mechanical homogenizers are more than capable of providing the same level of cell lysis in a fraction of the time it takes a bead mill.
Most mechanical homogenizing is done in a matter of seconds with ZERO heat produced and you can continue on with your same tubes you are processing in, no need to buy special tubes and beads!
The same piece of PRO homogenizing equipment can homogenize both soft and hard tissue samples.
What types of samples can they process?
What tubes can I use with each homogenizing method?
BeadBeater
Operating Instructions General
Monitoring cell lysis. The BeadBeater will disrupt over 90% of the cells in about 2-5 minutes of operation. The homogenization procedure involves cell ‘cracking’ action rather than high shear. After homogenization, cell membranes may still appear to be intact when viewed under a microscope. Therefore, to monitor the time course of cell breakage, rely on assay methods that measure intracellular constituents (e.g., OD260, enzyme activity, protein staining, PAGE). If the goal is to isolate intact intracelluar organelles, use the same size beads as suggested for cell disruption (see Beads). Beadbeating is a very effective for this purpose. To maximize the yield of intact organelles, homogenize for a shorter period of time to get about 70% maximum cell disruption. A homogenization time study of 1, 2, 3, and 5 minutes would be instructive.
Temperature control. The homogenate will be warm after three minutes of ‘BeadBeating’. When isolating proteins, membranes or organelles, cooling may be necessary. When isolating nucleic acids in aggressive extraction media containing phenol/chloroform, guanidinium salts, and/or detergents, temperature control is usually not necessary. The easiest way to minimize heating is by operating the BB, with the ice water jacket installed, for one minute and then let the homogenate sit for one minute, cycling thus until homogenization is complete. Also, consider replacing the clear polycarbonate chamber with a stainless steel closed chamber (accessory, #60801) for much better heat transfer to the ice water. For an even more stringent cooling technique see Methods in Enzymology, Vol.182, p.162-164.
Bead size selection. The correct size beads are 0.1 mm dia. for bacteria, 0.5 mm dia. for yeast, and 1.0 mm dia. or 2.5 mm dia. for chopped-up plant or animal tissue. While glass bead media is most commonly used, denser bead media is available for tough materials. Additional information on bead media is located at Beads.
Typical Operating Conditions
1) Fill the large, clear container or chamber 1/2 full with ice-cold beads and the rest of the volume with cells suspended in cold extraction media. Using the standard large polycarbonate chamber, that would be about 200 ml of beads and 200 ml of buffer containing 1-80 g wet weight of cells. Homogenizing cells at low concentrations is okay but, in the interest of efficient down-stream purification, it may be better to keep cell extract concentrations high by using a Small Chamber (accessory, #110803) designed to process 15 or 50 ml of cell suspension. Lay the large black rubber gasket on the the top lip of the filled chamber and gently lower the rotor assembly into the filled, clear plastic chamber. It is important to leave as little air as possible in the filled chamber. Holding the chamber/gasket/rotor assembly in one hand and the ice-water jacket (held up-side-down) in the other hand, firmly screw them together. If temperature control is not a concern, use the plastic, threaded Ring (it looks like the outer ring a thick Mason jar lid) in place of the ice-water jacket to seal the filled chamber.
2) Invert the whole assembly, fill the ice water jacket with crushed ice and water and place the assembly on the BeadBeater motor base. The bead-chain attached to the side of the motor base is only used to hold down the filled Large Chamber when it is sealed with the plastic, threaded Ring.
3) Run the BeadBeater for about three minutes (5 min. maximum). For cooling considerations, see Temperature Control comments above.
4) The homogenate can be recovered by simply decanting. To recover the entire product, one can either pour the homogenate, beads and all, into a Buchner funnel containing filter paper, and suction the homogenate out of the beads or one can attach a glass tube with a sintered glass tip (commonly used to aerate cultures) to a side arm flask and suck out the homogenate directly from the chamber.
Cleaning
Wash the beads with water and then detergent. Rinse the beads repeatedly with DI or RO water and dry them overnight at 50 deg C in a shallow glass or stainless steel tray. Properly cleaned beads will be flow freely. If some of the beads cake on the sides of the drying container, repeat the cleaning process. Beads can be autoclaved or baked, if desired. If you want beads free of all nucleic acids or nucleases, soak the beads for 5 minutes in a 1:10 dilution of ordinary household bleach solution in place of the detergent wash. Beads can be reused about ten times.
Do not let a ‘dirty’ chamber sit around. Residual cell homogenate is corrosive and will lead to jamming and leaking of the chamber. Hand wash the plastic rotor assembly and chamber promptly.
Things Not To Do
Do Not fill the polycarbonate chamber more than ¾ full with glass or ceramic beads. Sample heating will be excessive and the motor may burn out. On the other hand, the chamber must be at least ½ full of beads in order to get good cell disruption.
Do Not use beads larger than 2.5 mm diameter with the Large Chamber nor larger than 1mm diameter with the 15 ml or 50 ml Small Chamber. Steel beads cannot be used because they are too heavy to be agitated.
Do Not use larger vessels (Mason jars, etc.) with the BeadBeater. These containers do not achieve good homogenization and, if made of glass, may break and cause injury. BioSpec Products has extensive experience with the use of continuous bead-mills capable of processing multi-liter quantities of cell suspension. We would be happy to share our experience with you.
Do Not use flammable solvents in the chamber. The polycarbonate plastics in the chamber may be attacked. Furthermore, sparks from the BeadBeater brush-motor might ignite leaking solvent or fumes.
Cleaning BeadBeater Chambers
There is a method for thorough cleaning of BeadBeater chambers. It only takes a minute more than simply washing out the intact chamber but will assure that chamber components last longer. The rotor/shaft part of the BB homogenization chamber is temporarily removed from its black plastic bushing unit. By doing so, you will remove any abrasive glass fragments that might have worked their way into the shaft and bushing area:
Bead mill homogenizer
Sample cooling before, during and after milling
For the optimized SpeedMill PLUS sample holder different temperatures can be freely chosen due to the storage down to –80 °C. This warrants an efficient sample cooling during the whole lysis process and prevents the substantial sample warming that occurs with other bead beating homogenizers.
Liquid nitrogen or dry ice are no longer required, saving you the considerably expense of these additives.
키워드에 대한 정보 bead beater homogenizer
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