당신은 주제를 찾고 있습니까 “cy3 nhs ester – Antibody Conjugates: What You Need to Know | CST Tech Tips“? 다음 카테고리의 웹사이트 https://chewathai27.com/you 에서 귀하의 모든 질문에 답변해 드립니다: https://chewathai27.com/you/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 Cell Signaling Technology, Inc. 이(가) 작성한 기사에는 조회수 4,519회 및 좋아요 81개 개의 좋아요가 있습니다.
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Antibodies that have been conjugated to labels can aid the design of your experiments, but it’s important to understand the potential impact conjugation could have on antibody specificity and performance.
💡 Get insights and advice on techniques, publishing, and navigating a scientific career: https://www.cellmentor.com/
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ℹ️ Questions about an antibody, antibody conjugate, or protocol? Get in touch with a CST scientist: https://cellsignal.com/support
Partial transcript:
Have a look at the data on the left. What do all these images have in common? They were all generated using conjugated antibodies. Many immunoassays incorporate antibody conjugates, which are antibodies directly crosslinked to labels such as fluorescent dyes or proteins, enzymes, beads, or DNA. These labels can enable readout of the assay, multiplexed readouts, or a shorter experimental protocol.
If you decide your experiment requires an antibody conjugate, you’ll be looking through your lab’s inventory and searching on the web to make a shopping list of antibody conjugates that react to the proteins you’re interested in. So let’s say that you find an unconjugated antibody clone shown to be specific for your target, meaning the validation data was generated in your application of interest, using a relevant biological model. One might assume that conjugates of the same clone would have the same specificity and performance. Unfortunately, this is not always the case.
In this video, I’ll focus on examples of antibodies that are conjugated to fluorescent dyes for use in immunocytochemistry, flow cytometry, or other assays with fluorescent readouts. The validation principles I’ll describe here also apply to other types of conjugates, such as antibodies coupled to biotin, beads, or enzymes such as HRP.
The conjugation reaction involves direct, covalent modification of the amino acid side chains in the antibody. Various coupling chemistries are available that form a stable, covalent bond between the antibody and the desired label, in this case, a fluorescent dye. Two of the most commonly used chemistries, succinimidyl ester and maleimide, will target amino and thiol groups, respectively.
Unfortunately, conjugation also has the potential to alter the antibody’s structure or disrupt its function, meaning the antibody-antigen interactions could be affected. There’s also the potential for increased off-target binding and lower stability. The impact on specificity, performance and stability is highly dependent on the antibody, the label, and the conjugation chemistry.
That’s why it’s important to scrutinize the conjugates on your list to look for validation testing performed in the assay or application you will be using, and not rely solely on data from the unconjugated version of the antibody.
The example on the right shows an antibody conjugated with APC that failed to perform in flow cytometry; compare to the unconjugated Phospho-Stat5 antibody, detected with a secondary in the left panel. This particular APC-conjugated antibody failed our validation testing and was not released as a product.
Fortunately, by switching to a different fluorophore, phycoerythrin, we were able to produce a Phospho Stat5 antibody conjugate that passed validation testing.
And remember, for both conjugates and non-conjugates, validation performed in one type of immunoassay, such as western blot, is not sufficient to validate its performance in a different assay such as immunocytochemistry.
About CST®: Cell Signaling Technology (CST) is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools to the biomedical research community. Our employees operate worldwide from our U.S. headquarters in Massachusetts, and our offices in the Netherlands, China, and Japan. https://cellsignal.com/about
All trademarks are the property of their respective owners. For the most up-to-date trademark information, please visit https://www.cellsignal.com/trademarks
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cy3 nhs ester 주제에 대한 자세한 내용은 여기를 참조하세요.
Cyanine3 NHS ester, Yellow emitting fluorescent dye … – Abcam
Yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Analog of Cy3® NHS ester. Ideal for labeling soluble proteins, peptes, …
Source: www.abcam.com
Date Published: 2/30/2022
View: 4515
Cy3 NHS ester, 1393363-07-9 | BroadPharm
Cy3 NHS ester is a reactive cyanine dye containing NHS group which enables labeling of amino-bearing biomolecules. This reagent is eal for the labeling of …
Source: broadpharm.com
Date Published: 7/21/2021
View: 6786
Cy3 NHS Ester – Click Chemistry Tools
Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye.
Source: clickchemistrytools.com
Date Published: 11/9/2022
View: 1600
Cy3 NHS Ester – Fluoroprobes
Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye.
Source: fluoroprobes.com
Date Published: 8/18/2022
View: 1908
Cy3 NHS ester – Kerafast
Cy3 NHS ester is a reactive dye for the labeling of amino-groups in peptes, proteins, and oligonucleotes. This dye requires use of organic co-solvent (such …
Source: www.kerafast.com
Date Published: 3/28/2021
View: 1288
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- Author: Cell Signaling Technology, Inc.
- Views: 조회수 4,519회
- Likes: 좋아요 81개
- Date Published: 2021. 4. 30.
- Video Url link: https://www.youtube.com/watch?v=IKZogULZrqw
Cyanine3 NHS ester
Cyanine3 NHS ester
Cat. # Quantity Price Lead time Buy this product 11020 1 mg 110.00$ in stock Add to cart 21020 5 mg 210.00$ in stock Add to cart 41020 25 mg 410.00$ in stock Add to cart 51020 50 mg 695.00$ in stock Add to cart 61020 100 mg 1190.00$ in stock Add to cart Bulk inquiry Quantity * Salutation Full name * Company Phone * Email * Сomment Send request Delivery dates and prices will be calculated based on your ZIP Select country… Afghanistan American Samoa Australia Azerbaijan Bahrain Bangladesh Bhutan British Indian Ocean Territory Brunei Cambodia China Christmas Island Cocos (Keeling) Islands Cook Islands Fiji French Polynesia Guam Hong Kong SAR China India Indonesia Israel Japan Jordan Kiribati Kuwait Laos Lebanon Macao SAR China Malaysia Maldives Marshall Islands Micronesia Mongolia Myanmar (Burma) Nauru Nepal New Caledonia New Zealand Niue Norfolk Island Northern Mariana Islands Oman Pakistan Palau Palestinian Territories Papua New Guinea Philippines Qatar Samoa Saudi Arabia Singapore Solomon Islands South Korea Sri Lanka Syria Taiwan Tajikistan Thailand Timor-Leste Tokelau Tonga Turkmenistan Tuvalu U.S. Outlying Islands United Arab Emirates Uzbekistan Vanuatu Vietnam Wallis & Futuna Yemen Пожалуйста, укажите название города кириллицей I cannot find my country in this list Free express shipping from 300$ ! Add products for to your cart and get free express shipping Your cart total is 0$ , and your order qualifies for free express shipping to , ZIP . Delivery options for stock items to , ZIP Change country or ZIP Free express shipping from 300$ ! Add products for to your cart and get free express shipping to , ZIP . Free express shipping from 300$ ! Your cart total is 0$ , and your order qualifies for free express shipping to , ZIP . Change country or ZIP
Cyanine3 NHS ester is a reactive dye for the labeling of amino-groups in biomolecules, an analog of Cy3® NHS ester. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. For delicate proteins consider using water-soluble sulfo-Cyanine3 NHS ester which does not require use of any co-solvent.
Cyanine3 NHS ester is a replacement for NHS esters of Cy3® and DyLight 549.
Absorption and emission spectra of Cyanine3 fluorophore
Customers also purchased with this product Sulfo-Cyanine7 maleimide Water soluble near infrared dye maleimide for the labeling of proteins and peptides. Water soluble near infrared dye maleimide for the labeling of proteins and peptides. 1 mg – 110.00$ 5 mg – 290.00$ 25 mg – 690.00$ 50 mg – 1270.00$ 100 mg – 1990.00$ Add to cart Add this product to your cart and
get free express delivery Cyanine5.5 maleimide Cyanine5.5 maleimide is a thiol reactive dye for protein labeling. Cyanine5.5 maleimide is a thiol reactive dye for protein labeling. 1 mg – 110.00$ 5 mg – 210.00$ 25 mg – 410.00$ 50 mg – 695.00$ 100 mg – 1190.00$ Add to cart Add this product to your cart and
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General properties Appearance: red powder Mass spec M+ increment: 474.2 Molecular weight: 641.5 CAS number: 2632339-91-2 Molecular formula: C 34 H 40 N 3 BF 4 O 4 IUPAC name: 3H-Indolium, 2-[3-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3,3-dimethyl-, tetrafluoroborate Solubility: poorly soluble in water (2.3 mM = 1.5 g/L), soluble in organic solvents (DMF, DMSO, dichloromethane) Quality control: NMR 1H and HPLC-MS (95+%) Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. MSDS: Download Product specifications
Spectral properties Excitation/absorption maximum, nm: 555 ε, L⋅mol−1⋅cm−1: 150000 Emission maximum, nm: 570 Fluorescence quantum yield: 0.31 CF 260 : 0.04 CF 280 : 0.09
Product citations Wang, S.; Latallo, M.J.; Zhang, Z.; Huang, B.; Bobrovnikov, D.G.; Dong, D.; Livingston, N.M.; Tjoeng, W.; Hayes, L.R.; Rothstein, J.D.; Ostrow, L.W.; Wu, B.; Sun, S. Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD. Nature Communications, 2021, 12(1), 4908. doi: Nuclear export and translation of circular repeat-containing intronic RNA in C9ORF72-ALS/FTD., 12(1), 4908. doi: 10.1038/s41467-021-25082-9 Chiou, A.E.; Hinckley, J.A.; Khaitan, R:; Varsano, N.; Wang, J.; Malarkey, H.F.; Hernandez, C.J.; Williams, R.M.; Estroff, L.A.; Weiner, S.; Addadi, L.; Wiesner, U.B.; Fischbach, C. Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments. Small, 2021, 17(15), 2001432. doi: Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments., 17(15), 2001432. doi: 10.1002/smll.202001432 Chudinov, A.V.; Vasiliskov, V.A.; Kuznetsova, V.E.; Lapa, S.A.; Kolganova, N.A.; Timofeev, E.N. Mononucleotide repeat expansions with non-natural polymerase substrates. Scientific Reports, 2021, 11, 2423. doi: Mononucleotide repeat expansions with non-natural polymerase substrates., 11, 2423. doi: 10.1038/s41598-021-82150-2 Huo, S.; Zhao, P.; Shi, Z.; Zou, M.; Yang, X.; Warszawik, E.; Loznik, M.; Göstl, R.; Herrmann, A. Mechanochemical bond scission for the activation of drugs. Nature Chemistry, 2021, 13, 131–139. doi: Mechanochemical bond scission for the activation of drugs., 13, 131–139. doi: 10.1038/s41557-020-00624-8 Show more (85)
Cy® is a trademark of Cytiva.
Cyanine3 NHS ester, Yellow emitting fluorescent dye (ab146452)
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Cy3 NHS ester, 1393363-07-9
Cy3 NHS ester
Cy3 NHS ester is a reactive cyanine dye containing NHS group which enables labeling of amino-bearing biomolecules. This reagent is ideal for the labeling of soluble proteins, peptides, and oligonucleotides/DNA. Cyanine3 NHS ester is an analog of Cy3ｨ NHS ester and can replace Alexa Fluor 546 as well as DyLight 549.
Cy3 NHS Ester
Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Cy3 NHS ester is not recommended for labeling proteins at high molar ration due significant self-quenching, and only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 555 NHS Ester, which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
Cy3 is spectrally is almost identical to Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.
Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific.
Cy3 NHS ester
Cy3 NHS Ester (also sold under Sulfo-Cyanine3 NHS ester name) is a bright, water-soluble, and pH insensitive from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Cy3 NHS ester is not recommended for labeling proteins at high molar ration due significant self-quenching, and only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 555 NHS Ester (Alexa Fluor ® 555 analog), which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
Cy3 NHS ester (Cyanine3 NHS ester)
In Vitro:
DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)
Preparing
Stock Solutions Concentration Solvent Mass 1 mg 5 mg 10 mg 1 mM 1.6945 mL 8.4724 mL 16.9448 mL 5 mM 0.3389 mL 1.6945 mL 3.3890 mL 10 mM 0.1694 mL 0.8472 mL 1.6945 mL
* Please refer to the solubility information to select the appropriate solvent.
In Vivo:
1. Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution
2. Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline) Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution
Cy3 NHS ester
Cy3 NHS ester is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
This dye requires use of organic co-solvent (such as DMF, or DMSO) for the labeling. Water-soluble Cy3 is also available.
NHS Ester Labeling of Amino Biomolecules
키워드에 대한 정보 cy3 nhs ester
다음은 Bing에서 cy3 nhs ester 주제에 대한 검색 결과입니다. 필요한 경우 더 읽을 수 있습니다.
이 기사는 인터넷의 다양한 출처에서 편집되었습니다. 이 기사가 유용했기를 바랍니다. 이 기사가 유용하다고 생각되면 공유하십시오. 매우 감사합니다!
사람들이 주제에 대해 자주 검색하는 키워드 Antibody Conjugates: What You Need to Know | CST Tech Tips
- Antibody conjugation
- antibody modification
- conjugation reaction
- fluorescent antibody
- antibody conjugate
- antibody labeling
- antibody labelling
- covalent antibody
- antibody cross-linking
- antibody coupling
- alexa fluor conjugate
- flow cytometry
- immunofluorescence
- DIY conjugation
- FACS antibody
- yt:cc=on
- antibody validation
Antibody #Conjugates: #What #You #Need #to #Know #| #CST #Tech #Tips
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