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ProntoSIL HPLC columns from Bischoff Chromatography are available in a wide variety of bonded phases and dimensions. In addition to more typical C8 and C18 phases, ProntoSIL columns are also available in hard to find bonded phases such as C1, amino, DIOL and C30.
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d여기에서 How to choose the right HPLC column – AND – favorite HPLC columns from Agilent, Waters \u0026 Phenomenex – prontosil hplc column 주제에 대한 세부정보를 참조하세요
How do I choose the right HPLC column?
So Step 1 – look in a column catalog. It’s gonna give you a guaranteed result.
OK let’s say that you don’t have a column catalog. Or let’s say that your chemical isn’t in one. Let’s say that you are sitting there saying, “Well Lee if I knew all of that I wouldn’t be asking you for advice.”
So that is a valid point. Let me take you to the next step.
In HPLC we have dozens of columns out there, but there is one column that dominates. If you look at all the column sales, about 85% of the column sales are reversed phase columns.
Why is that? To me that means that 85% of the separation problems must be solved by reversed phase. Not every one, 15% won’t be. But let’s play the odds.
What you want to do is to choose one really good reversed phase column. You want to buy the best base-deactivated C-18 column that you can get.
C-18 is going to be your most useful reversed phase column. There are dozens of different C-18 columns out there. What you want to find is the most modern C-18 column out there.
So here is a weird thing. When you look in the catalog they are going to list their oldest columns and their newest columns. And catalogs won’t really tell you that their old columns are lousy. That catalog – I hate to say it – is filled with lousy columns.
It doesn’t mean that Agilent makes lousy columns, it means that there are old-fashioned columns that they still make for old-fashioned applications.
If you are starting a new application you want to start with today’s best technology.
So my favorite column is eclipse plus made by Zorbax, by Agilent. There are have their SB-C18 that is also fantastic it is their #1 selling column. I personally like the Eclipse Plus.
But I won’t limit it to my Agilent friends. Waters make a couple of great columns. Waters SunFire, a beautiful column, a beautiful C18 base deactivated column. They also make one called XBridge. A different technology, very high-pH stable, an excellent column.
My friends at Phenomenex make a column called the Luna C18, the #1 selling silica in the world. There is a newer version called the Gemini C18 – I don’t like the cute names but I like the column. The Gemini C-18 is the latest and the greatest out of Phenomenex.
I feel bad for all the companies that I did not mention. There are other great ones out there.
But you start with a good base deactivated C18 column. My next piece of advice is you buy a column with the most efficiency you can get. We haven’t defined efficiency yet. Come back to one of my lectures and I will define efficiency for you. But it is a measure of the skinniness of the peak.
So when you are entering method development I have no idea how much efficiency that I need, so let’s start with the longest with the most efficiency. If that works and I have empty space I can go to a shorter column to save time. But if I start with a short column, it may not work and then I would have to go to a long column. So in other words, start with a really long, really high efficiency base-deactivated C18 column. In my world that would mean a 10 cm/1.8 micron, or a 15 cm/3.5 micron, or a 25 cm/5 micron – old fashioned!
Any of those are brute force columns.
So to summarize: good c18 base deactivated, high efficiency. Start with that. That one column will handle most of the applications out there in the reversed phase world.
So there’s your go-to guide for choosing the right column in HPLC
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ProntoSIL HPLC Columns
Another column, ProntoSIL C18-EPS, uses dual siloxane bonds to increase bonded phase stability and actually demonstrates much greater durability than other C18 …
Source: www.hplc.eu
Date Published: 11/18/2021
View: 7003
ProntoSIL® C18-EPS Reversed Phase HPLC Column, MAC …
ProntoSIL® C18-EPS is a high performance base deactivated column with enhanced polar selectivity specifically designed for high resolution separation of …
Source: us.vwr.com
Date Published: 7/17/2021
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Prontosil HPLC Columns -Bischoff Germany – IndiaMART
Prontosil HPLC Columns -Bischoff Germany … Imptech Scientific a pioneer in HPLC and GC columns is associated with Bischoff Chromatography for their complete …
Source: www.indiamart.com
Date Published: 11/20/2021
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ProntoSIL 120-5 C18 SH, Column 250 x 4.6 mm – KNAUER
ProntoSIL C18 SH is the stationary phase in the ProntoSIL line with the highest carbon load. It is fully endcapped. Due to the carbon load it exhibits an …
Source: www.knauer.net
Date Published: 2/13/2022
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ProntoSIL C18-EPS Reversed-Phase HPLC Columns
A major cause of column failure is the loss of bonded phase from the silica support due to hydrolysis of the siloxane bond. ProntoSIL C18-EPS uses a unique,.
Source: www.obrnutafaza.hr
Date Published: 6/28/2022
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주제와 관련된 더 많은 사진을 참조하십시오 How to choose the right HPLC column – AND – favorite HPLC columns from Agilent, Waters \u0026 Phenomenex. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 prontosil hplc column
- Author: Axion Labs
- Views: 조회수 1,792회
- Likes: 좋아요 49개
- Date Published: 2021. 8. 28.
- Video Url link: https://www.youtube.com/watch?v=9j8hWVY2neY
Prontosil HPLC columns
German quality…
ProntoSIL HPLC columns from Bischoff Chromatography are available in a wide variety of bonded phases and dimensions. In addition to more typical C8 and C18 phases, ProntoSIL columns are also available in hard to find bonded phases such as C1, amino, DIOL and C30.
Phase Functional group Pore, A Particle, um Carbon, % pH range Use
Original ProntoSIL columns
ProntoSIL Si Silica 60/120/200/300 3/5/10 — — ProntoSIL is the silica support of BISCHOFF Chromatography. It can be used in normal phase chromatography.
ProntoSIL C18 ace-EPS C18 60/120/200/300 3/5/10 18.5 – 8.5 1 – 10 ProntoSIL C18 ace-EPS belongs to the new group of stationary RP-supports with polar embedded groups. This packing offers a maximum of hydrophobicity combined with a maximum of polar selectivity. The silanophilic activity of the support is very low. Ultra strong basic compounds like amitriptyline can be eluted from the column in neutral pH values with excellent symmetrical peak shapes.
ProntoSIL C18 AQ C18 120/200 3/5/10 14 – 9 3 – 8 Used in aqueous mobile phases with an organic content below 10%.
ProntoSIL C18 AQ PLUS C18 120 3/5/10 17 3 – 8 ProntoSIL C18 AQ PLUS shows an enhanced stability at low pH’s down to pH 1. Also the packing shows excellent peak shapes in pure aqueous eluents but differs in shape selectivity compared to ProntoSIL C18 AQ. The application field for this support is mainly in combinatorial chemistry where the standard separation conditions are fast gradients from 0 – 100% organic and where the mobile phases include 0.1% TFA.
ProntoSIL C18 SH C18 120 3/5/10 17 1 – 8 This phase is fully endcapped. Due to the carbon load it shows an excellent shape selectivity and stability even at pH 1.
ProntoSIL C18 H C18 60/120/200/300 3/5/10 18.5 – 7 3 – 8 ProntoSIL C18 H is the standard C18 support of BISCHOFF Chromatography. It is applicable in a wide range of RP- chromatography. The packing is fully endcapped and shows all of the excellent properties a new generation stationary phase can offer.
ProntoSIL C8 ace-EPS C8 120/300 3/5 12 – 9 3 – 8 In comparison to ProntoSIL C18 ace-EPS, ProntoSIL C8 ace-EPS shows an even higher polar selectivity. Due to the shorter alkyl chain the influence of the polar group is increased.
ProntoSIL C8 SH C8 60/120/200/300 3/5/10 12 – 4 1 – 8 ProntoSIL C8 SH is a classical C8-type stationary phase; it is fully endcapped. Due to the bonding technology it shows an excellent shape selectivity and stability even at pH 1. The 5um support is also available in 60A. This packing shows an enhanced selectivity comparable to Lichrospher Select B from Merck.
ProntoSIL C4 C4 60/120/200/300 3/5 7.5 – 2.5 1 – 8 ProntoSIL C4 is a classical C4-type stationary phase; it is fully endcapped. Due to the bonding technology it shows an enhanced stability even at pH 1. The C4-packings show excellent properties for the separation of large biomolecules like proteins and peptides not only in the RP-mode but also in the HIC-mode.
ProntoSIL C1 C1 120 3/5 3 1 – 8 The C1 packing shows the lowest retention of the complete ProntoSIL line. The application area is mainly the separation of non-polar solutes. It can also be used for the separation of proteins in the HIC-mode. Due to the bonding technology the C1-bonding type is stable down to pH 1.
ProntoSIL C30 C30 120/200/300 3/5/10 25 – 13 1 – 8 ProntoSIL C30 is a stationary phase with a high carbon load. The high coverage of the support results in a very dense bonding and in an excellent shape selectivity and stability even at pH 1. Especially the wide pore supports are showing an enhanced shape selectivity. The application field of the C30 packings is the separation of isomers of carotenoids and other long alkyl chain solutes that can not be separated on classical C18 columns.
ProntoSIL Phenyl Phenyl 60/120 3/5 12 – 9.5 1 – 8 ProntoSIL Phenyl is a RP packing that offers different selectivities in comparison to brush type stationary phases like C18 or C8; it is fully endcapped. Due to the bonding technology it shows excellent stability even at pH 1.
ProntoSIL Amino NH2 120 3/5/10 4 3 – 8 This phase can be used in three modes: NP-mode, RP-mode and IC-mode. In the NP-mode it is an alternative to the silica packings but offers different selectivities. In the RPmode the packing is mainly used for carbohydrate analysis. In the IC-mode the bonded phase can be used as a weak anion exchanger (WAX) for the analysis of anions and organic acids. The packing shows excellent properties in Supercritical Fluid Chromatography (SFC) where dense gases (i.e. CO2) are used as mobile phases.
ProntoSIL Amino E NH2 120 5 5 3 – 8 In comparison to ProntoSIL Amino this support is fully endcapped. Due to this, it is perfectly suitable for the SFC of basic compounds.
ProntoSIL Amino H NH2 120 5 4.5 3 – 8 This packing shows a different selectivity in Supercritical Fluid Chromatography (SFC). Due to a special bonding technology it shows a higher lifetime in carbohydrate analysis in the RP-mode.
ProntoSIL CN CN 120 3/5 6 – 5 3 – 8 In RP-mode, the application area of ProntoSIL CN is the separation of strong basic solutes. In NPmode, the ProntoSIL CN-support offers a complementary selectivity to the other NP-phases: Silica, Amino and DIOL. Due to the quick equilibration time of the CN-bonded phase it is the best choice for gradient elution in NPmode.
ProntoSIL OH DIOL 60/120 3/5/10 5.5 – 4 3 – 8 ProntoSIL OH is a DIOL bonded phase. The DIOL packing can be used in Normal Phase (NP) and Reversed Phase (RP) Chromatography. It is also suitable for Supercritical Fluid Chromatography (SFC). The silanophilic activity is lower than that of pure silica. This is particularly advantageous in Size Exclusion Chromatography (SEC). When the DIOL phase is employed in NP mode, the equilibration times are shorter than on a pure silica support, the selectivities, however, are similar.
ProntoSIL Enviro PHE proprietary 120 3 9.5 3 – 8 ProntoSIL Enviro PHE is a special stationary phase for the determination of Phenols in environmental analysis. This stationary phase was designed to meet the requirements of EPA method 604/625. In this procedure 11 phenols have to be separated. The columns are pretested with a standard mixture described in EPA method 604/625.
ProntoSIL Enviro PTL proprietary 60 5 2.5 3 – 8 ProntoSIL Enviro PTL is a special stationary phase for the group determination of non-aromatic, monoaromatic, diaromatic and polyaromatic hydrocarbons in petroleum products. This stationary phase was designed to meet the requirements of European Norm prEN 12916 (June 1997). In this norm a resolution between Cyclohexane (non-aromatic) and o-Xylene (monoaromatic) of minimum 7 is required. Therefore the columns packed with this stationary phases are only offered in the length of 250 mm. The columns are pretested with a standard mixture described in prEN 12916.
Enviro PES proprietary 100 5 15 3 – 8 ProntoSIL Enviro PES is a special stationary phase for the determination of pesticides in environmental analysis. This stationary phase was designed to meet the requirements of German industry norm DIN 38407 F12. In this procedure 17 pesticides have to be separated. ProntoSIL Enviro PES columns are able to separate this complex mixture in less than 45 minutes. The columns are shipped pretested with the pesticide standard mixture.
PolyEncap A C4 120/300 3/5/7/10 8 – 4 3 – 10 PolyEncap stationary phases are a family of bonded phases where the silica is polymeric encapsulated. The encapsulation is done by acrylic acid ester polymers which are additionally covalent bonded to the silica surface. This type of stationary phase offers different selectivities compared to classical bonded brush type stationary phases. PolyEncap A has a comparable selectivity to a brush type C8 packing.
PolyEncap B C18 120 3/5 14 3 – 10 PolyEncap stationary phases are a family of bonded phases where the silica is polymeric encapsulated. The encapsulation is done by acrylic acid ester polymers which are additionally covalent bonded to the silica surface. This type of stationary phase offers different selectivities compared to classical bonded brush type stationary phases. PolyEncap B has a comparable selectivity to a brush type C18 packing.
Commercially equivalent phases
Hypersorb ODS C18 120 3/5 9.5 3 – 8 ProntoSIL Hypersorb ODS is the recommended alternative for Hypersil ODS . It is based on a type A silica and has the same bonding density like the original RP support. Due to a slightly lower acidy of the silica the silanophilic activity of the packing is a little bit lower compared to Hypersil ODS. The polarity of the surface is comparable. In the application of these stationary phases the same selectivity but better peak shape for the elution of basic compounds are observed.
Spheribond ODS 1 C18 80 3/5/10 7 3 – 8 ProntoSIL Spheribond ODS 1 is the recommended alternative for Waters Spherisorb ODS 1 . It is based on a type A silica and has the same physical chemical properties and bonding density like the original RP support. The selectivity of the two packings is the same.
Spheribond ODS 2 C18 80 3/5/10 12 3 – 8 ProntoSIL Spheribond ODS 2 is the recommended alternative for Waters Spherisorb ODS 2 . It is based on a type A silica and has the same physical chemical properties and bonding density like the original RP support. The selectivity of the two packings is the same. The typical application field of this classical packing with moderate silanophilic activity is pharmaceutical analytes. The Waters Spherisorb ODS 2 column is the most popular spherical silica support in pharmaceutical applications.
KromaPlus C18 C18 110 3.5/5/10 20 1 – 10 ProntoSIL KromaPlus – the Kromasil ODS alternative – is based on ProntoSIL Silica and is available for analytical and preparative HPLC. Synthesized with our special bonding technology, an ultra-pure phase with very high batch-to-batch reproducibility and high chemmical stability is obtained. The bonding type is monofunctional and the phase is end-capped.
HIPAK ODS AB C18 130 3/5 11 3 – 8 HIPAK is the typical second generation packing of Bischoff Chromatography. It is based on a type A silica but with additional base deactiviation. This packing is our recommended alternative to Hypersil BDS .
MZ-Analysentechnik GmbH
Prontosil™ is a family of HPLC columns, manufactured by Bischoff-Chromatography, Germany. The phases are based on high-purity, base-deactivated, spherical silica gel. Many different pore sizes and modifications are available. Prontosil™ Enviro HPLC columns are especially designed for issues connected with environmental analysis.
ProntoSIL® C18-EPS Reversed Phase HPLC Column, MAC-MOD Analytical
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ProntoSIL 120-5 C18 SH, Column 250 x 4.6 mm
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