Top 23 Sun Gel Staining Solution The 189 Detailed Answer

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sun gel staining solution

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• Summary of article content: Articles about sun gel staining solution sun-gel staining solution … Ethium Brome Adsorber – CAS 69011-20-7 – Calbiochem Developed specifically for the. Ethium Brome … …
• Most searched keywords: Whether you are looking for sun gel staining solution sun-gel staining solution … Ethium Brome Adsorber – CAS 69011-20-7 – Calbiochem Developed specifically for the. Ethium Brome …
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sun gel staining solution

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• Summary of article content: Articles about sun gel staining solution SUN GEL Staining Solution은 전기영동(SDS-PAGE) 후 polyacrylame gel 상의 단백질을 염색하는 시약으로서 De-staining 과정없이 깨끗하게. 단백질 밴드를 염색, 관찰할 … …
• Most searched keywords: Whether you are looking for sun gel staining solution SUN GEL Staining Solution은 전기영동(SDS-PAGE) 후 polyacrylame gel 상의 단백질을 염색하는 시약으로서 De-staining 과정없이 깨끗하게. 단백질 밴드를 염색, 관찰할 …
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EZ-Gel Staining Solution | ITSBio

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• Summary of article content: Articles about EZ-Gel Staining Solution | ITSBio EZ (CBB) Gel Staining Solution은 단백질 전기영동 후, polyacrylame gel 상의 단백질을 염색할 때 사용하는 시약으로, CBB G250의 단점을 보완하여 빠른 시간 내에 … …
• Most searched keywords: Whether you are looking for EZ-Gel Staining Solution | ITSBio EZ (CBB) Gel Staining Solution은 단백질 전기영동 후, polyacrylame gel 상의 단백질을 염색할 때 사용하는 시약으로, CBB G250의 단점을 보완하여 빠른 시간 내에 … EZ-Gel Staining Solution
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MPs | Free Full-Text | Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels | HTML

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• Summary of article content: Articles about MPs | Free Full-Text | Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels | HTML Heat the gel in the destaining solution in a microwave oven for 40 s and subsequently agitate at room temperature (~25 °C) for 30–60 min for complete destaining … …
• Most searched keywords: Whether you are looking for MPs | Free Full-Text | Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels | HTML Heat the gel in the destaining solution in a microwave oven for 40 s and subsequently agitate at room temperature (~25 °C) for 30–60 min for complete destaining … Coomassie brilliant blue (CBB) dyes have been commonly used for the staining of protein bands in polyacrylamide gel electrophoresis (PAGE) gels. However, the staining and destaining of CBB dyes are time-consuming, and the use of methanol is hazardous to one’s health. I introduce a rapid electrophoretic destaining method using a semi-dry transfer unit and a high current power supply. In this method, ethanol was used instead of the hazardous methanol. Most of the protein bands became visible in 30 min. After a secondary destaining step, residual CBB was completely destained. The detection limit for a tested protein (5 ng) was higher than that of the conventional method. Therefore, this method is superior in its speed, safety, low cost, and sensitivity.
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Abstract

1 Introduction

2 Experimental Design

3 Procedure

4 Expected Results and Discussion

5 Conclusions

6 Reagents Setup

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Conflicts of Interest

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Sản phẩm | EcoDye Nucleic Acid Staining Solution

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• Summary of article content: Articles about Sản phẩm | EcoDye Nucleic Acid Staining Solution It is a non-mutagenic, non-cytotoxic product that can be used to replace toxic ethium brome in agarose gel electrophoresis. EcoDye ™ Nucleic Ac Staining … …
• Most searched keywords: Whether you are looking for Sản phẩm | EcoDye Nucleic Acid Staining Solution It is a non-mutagenic, non-cytotoxic product that can be used to replace toxic ethium brome in agarose gel electrophoresis. EcoDye ™ Nucleic Ac Staining …
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dsGreen Gel Staining Solution, 10000× : LumiProbe Life Science Solutions

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• Summary of article content: Articles about dsGreen Gel Staining Solution, 10000× : LumiProbe Life Science Solutions Product namePrice / USD 10010dsGreen Gel Staining Solution, 10000×, … 8(10), e77666. doi: 10.1371/journal.pone.0077666Song, C.-X.; Sun, Y.; Dai, Q.; Lu, … …
• Most searched keywords: Whether you are looking for dsGreen Gel Staining Solution, 10000× : LumiProbe Life Science Solutions Product namePrice / USD 10010dsGreen Gel Staining Solution, 10000×, … 8(10), e77666. doi: 10.1371/journal.pone.0077666Song, C.-X.; Sun, Y.; Dai, Q.; Lu, … Order, Questions, Request a sample : [email protected] no.Product namePrice / USD　10010dsGreen Gel Staining Solution, 10000×, 0.5 mL100.00in stock20010dsGreen Gel Staining Solution, 10000×, 1 mL180.00in stockA0010dsGreen Gel Staining Solution, 10000×, 50 uL29.00in stockdsGreen, an equivalent of SYBR® Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels. A qPCR grade reagent is also available.Unlike ethidium bromide, dsGreen is highly selective towards double stranded DNA, much less harmful, and offers better sensitivity.Excitation and emission spectra of dsDNA complex with dsGreenSpectral propertiesExcitation/absorption maximum, nm:454ε, L⋅mol−1⋅cm−1:73000Emission maximum, nm:524Fluorescence quantum yield:0.8Product citationsChetverikov, P.E.; Craemer, C.; Cvrković, T.; Klimov, P.B.; Petanović, R.U.; Romanovich, A.E.; Sukhareva, S.I.; Zukoff, S.N.; Bolton, S.; Amrine, J. Molecular phylogeny of the phytoparasitic mite family Phytoptidae (Acariformes: Eriophyoidea) identified the female genitalic anatomy as a major macroevolutionary factor and revealed multiple origins of gall induction. Experimental & Applied Acarology, 2021, 83(1), 31–68. doi: 10.1007/s10493-020-00571-6Chetverikov, P.E.; Cvrković, T.; Efimov, P.G.; Klimov, P.B.; Petanović, R.U.; Romanovich, A.E.; Schubert, M.A.; Sukhareva, S.I.; Zukoff, S.N.; Amrine, J. Molecular phylogenetic analyses reveal a deep dichotomy in the conifer-inhabiting genus Trisetacus (Eriophyoidea: Nalepellidae), with the two lineages differing in their female genital morphology and host associations. Experimental and Applied Acarology, 2020, 81(3), 287–316. doi: 10.1007/s10493-020-00503-4Kirkham, C.M.; Scott, J.N.F.; Wang, X.; Smith, A.L.; Kupinski, A.P.; Ford, A.M.; Westhead, D.R.; Stockley, P.G.; Tuma, R.; Boyes, J. Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome Instability. Molecular Cell, 2019, 74(3), 584–597.e9. doi: 10.1016/j.molcel.2019.02.025Komarova, N.; Andrianova, M.; Glukhov, S.; Kuznetsov, A. Selection, Characterization, and Application of {ssDNA} Aptamer against Furaneol. Molecules, 2018, 23(12), 3159. doi: 10.3390/molecules23123159Zietzer, A.; Buschmann, E.E.; Janke, D.; Li, L.; Brix, M.; Meyborg, H.; Stawowy, P.; Jungk, C.; Buschmann, I.; Hillmeister, P. Acute Physical Exercise and Long-Term Individual Shear Rate Therapy Increase Telomerase Activity in Human Peripheral Blood Mononuclear Cells. Acta Physiologica, 2017, 220(2), 251–262. doi: 10.1111/apha.12820Diaz-Romero, J.; Kürsener, S.; Kohl, S.; Nesic, D. S100B+A1 CELISA: A Novel Potency Assay and Screening Tool for Redifferentiation Stimuli of Human Articular Chondrocytes. Journal of Cellular Physiology, 2017, 232(6), 1559–1570. doi: 10.1002/jcp.25682Richter-Heitmann, T.; Eickhorst, T.; Knauth, S.; Friedrich, M.W.; Schmidt, H. Evaluation of Strategies to Separate Root-Associated Microbial Communities: A Crucial Choice in Rhizobiome Research. Frontiers in Microbiology, 2016, 7, 773. doi: 10.3389/fmicb.2016.00773Jain, D.; Siede, W. Rad5 Template Switch Pathway of DNA Damage Tolerance Determines Synergism between Cisplatin and NSC109268 in Saccharomyces cerevisiae. PLoS ONE, 2013, 8(10), e77666. doi: 10.1371/journal.pone.0077666Song, C.-X.; Sun, Y.; Dai, Q.; Lu, X.-Y.; Yu, M.; Yang, C.-G.; He, C. Detection of 5-Hydroxymethylcytosine in DNA by Transferring a Keto-Glucose by Using T4 Phage beta-Glucosyltransferase. ChemBioChem, 2011, 12(11), 1682-1685. doi: 10.1002/cbic.201100278Song, C.-X.; Yu, M.; Dai, Q.; He, C. Detection of 5-hydroxymethylcytosine in a combined glycosylation restriction analysis (CGRA) using restriction enzyme Taq[alpha]I. Bioorganic & Medicinal Chemistry Letters, 2011, 21(17), 5075-5077. doi: 10.1016/j.bmcl.2011.03.118과학기자재 및 시약판매, 박성준대표
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EZ-Gel Staining Solution

Description

EZ (CBB) Gel Staining Solution은 단백질 전기영동 후, polyacrylamide gel 상의 단백질을 염색할 때 사용하는 시약으로, CBB G250의 단점을 보완하여 빠른 시간 내에 단백질을 검출할 수 있도록 만든 solution입니다.

Product details

Sample type Protein Assay type CBB stain solution Storage Condition 0 ~ 4 ℃, Protect from light Kit Components 1 L, 1 Bottle Advantages 빠른 시간 내에 단백질을 검출 할 수 있습니다.

(10분 이내 protein band 확인, 1 시간이면 충분합니다.

Very low background 제품이며, destain solution을 필요로 하지 않습니다.

Low nano-gram의 단백질도 detection이 가능합니다.

장시간 염색에도 over stain이 되지 않습니다.

Toxic한 유기 용매를 사용하지 않습니다. (No Methanol, No Acetic Acid)

여러 번 재사용이 가능하기 때문에 경제적입니다. Application SDS-PAGE, 2D electrophoresis 등에 사용되는 poly-acrylamide gel에 사용 가능합니다.

Data

Coomassie Brilliant Blue G250 (dye)가 단백질의 Arg, Lys이나 aromatic ring이 있는 아미노산 잔기들(Tyr, Trp, Phe, His)등과 결합하여 단백질을 육안으로 볼 수 있게 염색하여 확인합니다.

FAQ

Q1. 염색 전 gel을 D.W 등으로 washing 해야 하나요?

A. 아닙니다. 타사 제품들은 gel staining 전 염색 dye가 잘 붙을 수 있도록 SDS 제거를 위해 D.W(증류수) 등으로 washing 해야 하지만, DoGEN EZ Gel Stain Solution은 washing이 필요 없습니다. 오히려 washing 할 경우 band가 연해 질 수 있습니다.

Q2. 염색 후, gel matrix에 남아 있는 background를 제거하려면 어떻게 해야 하나요?

A. 충분히 염색 후, gel을 D.W에 담그기만 두셔도 충분히 background가 사라집니다. 빠르게 background를 제거 하실 때는 교반기 위에서 washing을 진행하시거나 destain solution을 이용하시면 빠르게 단백질 band를 확인 하실 수 있습니다.

Q3. 재사용이 가능한가요?

A. 네. 가능합니다. 하지만 여러 번 재 사용 시 감도가 약해질 수도 있습니다.

Q4. 염색 시간은 어떻게 되나요?

A. 보통 10분 정도면 단백질 band를 확인 할 수 있지만, 조금 더 진하게 염색된 결과를 얻기 위해서는 1 ~ 2 시간 정도 충분히 염색해 주시면 됩니다.

Q5. 오랜 시간 gel을 염색하게 되면 어떻게 되나요?

A. 단백질만 염색이 되기 때문에 3 ~ 4일 정도 gel을 DoGEN EZ Gel Stain Solution에 넣어 두셔도 over staining 되지 않습니다. 다만, 장시간 염색으로 인해 background가 진해지지만 band 식별에는 영향을 주지 않습니다.

Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels

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Polyacrylamide gel electrophoresis (PAGE) is one of the most widely used methods to analyze proteins biochemically [ 1 ]. After separation by electrophoresis, protein bands need to be detected with a subsequent staining step. A number of staining methods for PAGE gels have been reported, such as Coomassie brilliant blue (CBB), amido black, and silver staining [ 2 4 ]. Coomassie brilliant blue staining has been commonly used to stain protein bands in PAGE gels, due to its low cost, easy operation, and relatively high sensitivity [ 5 ]. However, according to the typical protocol, the staining and destaining steps require a long time (from 4 h to overnight). Several rapid staining and destaining methods with reduced processing times have been reported. Hervieu reported that CBB-stained sodium dodecyl sulfate (SDS)-PAGE gels could be destained by boiling in distilled water [ 6 ]. It has been reported that heat treatment accelerates the staining and destaining steps [ 7 8 ]. A modified, rapid Fairbanks Coomassie stain, using a microwave oven, gave a sensitivity limit of 5 ng [ 9 10 ]. However, boiling a solution containing methanol is hazardous to one’s health. Dong et al. reported CBB-staining and destaining at boiling temperature without acid and methanol [ 11 ]. Lawrence et al. reported a staining method using CBB G-250 with a low concentration of hydrochloric acid, instead of acetic acid [ 12 ]. In these procedures, the hot destaining solution should be exchanged repeatedly, leading to risk of burn injury. Kang et al. reported a sensitive colloidal staining method using CBB G-250, using less-toxic ethanol instead of methanol [ 13 14 ]. Although this staining method is very sensitive, up to ~1 ng, more than two hours of incubation is required for 80% staining. Several companies produce rapid protein staining/destaining systems, such as eStain™ from GenScript (Piscataway, NJ, USA, Cat. no.: L00657) or Pierce™ Power Stainer from Thermo Scientific (Waltham, MA, USA, Cat. no.: 22840). However, the electrophoretic apparatuses are expensive (~2000), and each staining costs ~5 per gel. Here, I introduce a rapid, safe, and less expensive method to stain and destain PAGE gels. In this method, PAGE gels are stained and destained electrophoretically using ethanol instead of methanol. Only one replacement of the boiled water is required. Most of the protein bands became visible in less than 30 min, and thus, it reduces the overall experimental time. Furthermore, the detection limit (5 ng) was lower than that of conventional methods (10 ng).

EcoDye Nucleic Acid Staining Solution

EcoDye ™ Nucleic Acid Staining Solution is a stable fluorescent dye that stains DNA / RNA. It is a non-mutagenic, non-cytotoxic product that can be used to replace toxic ethidium bromide in agarose gel electrophoresis. EcoDye ™ Nucleic Acid Staining Solution is strongly coupled with DNA or RNA to emit green fluorescence and can be detected by UV imaging or LED system.

FEATURES

Available as an alternative to EtBr (ethidium bromide)

High sensitivity

Non-toxic, Non-mutagenic, Non-carcinogenic

Both dsDNA, ssDNA and RNA can be identified

Completed various stability tests

(Ames test, micronucleus test, in vitro mammalian cell chromosomal aberration test)

STORAGE TEMPARATURE

EXPIRATION DATE

4 ℃

2 Years

(in case of storage at 4 ° C)

So you have finished reading the sun gel staining solution topic article, if you find this article useful, please share it. Thank you very much. See more: Coomassie Blue staining solution, SDS-PAGE Coomassie Blue staining, Coomassie blue staining 조성, 쿠마 시 블루 염색 프로토콜, CBB staining 원리, 쿠마 시 블루 염색 시간, Coomassie g250, G250 R250