당신은 주제를 찾고 있습니까 “quick blunting kit – Quick Tip: Blunting Nails“? 다음 카테고리의 웹사이트 Chewathai27.com/you 에서 귀하의 모든 질문에 답변해 드립니다: Chewathai27.com/you/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 Bob Vila 이(가) 작성한 기사에는 조회수 2,662회 및 좋아요 16개 개의 좋아요가 있습니다.
quick blunting kit 주제에 대한 동영상 보기
여기에서 이 주제에 대한 비디오를 시청하십시오. 주의 깊게 살펴보고 읽고 있는 내용에 대한 피드백을 제공하세요!
d여기에서 Quick Tip: Blunting Nails – quick blunting kit 주제에 대한 세부정보를 참조하세요
When nailing near the end of a piece of lumber, there’s a possibility of splitting the wood. Bob Vila explains how to minimize that problem.
quick blunting kit 주제에 대한 자세한 내용은 여기를 참조하세요.
Quick Blunting™ and Quick Ligation™ Kits – NEB
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into …
Source: www.nebiolabs.com.au
Date Published: 11/23/2022
View: 5492
Quick Blunting™ Kit #E1201 – NEB UK
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into …
Source: www.neb.uk.com
Date Published: 10/23/2021
View: 5040
New England Biolabs, Inc. Quick Blunting Kit – 20 reactions
The Quick Blunting Kit is used to convert DNA with incompatible 5′ or 3′ overhangs to 5′ phosphorylated, blunt-ended DNA for efficient blunt-end ligation …
Source: www.fishersci.com
Date Published: 12/25/2022
View: 3893
Quick blunting kit | Sigma-Aldrich
quick blunting kit … This cloning protocol includes selecting the cloning system and plasm vector, plasm restriction digestion, fragment restriction …
Source: www.sigmaaldrich.com
Date Published: 2/30/2021
View: 8369
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New …
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into …
Source: pr.vwr.com
Date Published: 11/12/2022
View: 6536
DNA Blunting Kit – Takara Bio
The DNA Blunting Kit allows the conversion of 3′ and 5′ overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3’→5′ …
Source: www.takarabio.com
Date Published: 8/24/2022
View: 7779
Quick Blunting Kit – New England Biolabs GmbH
Quick Blunting Kit, Article Number: E1201.
Source: www.neb-online.de
Date Published: 7/11/2022
View: 8488
주제와 관련된 이미지 quick blunting kit
주제와 관련된 더 많은 사진을 참조하십시오 Quick Tip: Blunting Nails. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 quick blunting kit
- Author: Bob Vila
- Views: 조회수 2,662회
- Likes: 좋아요 16개
- Date Published: 2011. 10. 28.
- Video Url link: https://www.youtube.com/watch?v=P2YRiUGg7CA
Quick Blunting™ and Quick Ligation™ Kits
Kit Components
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
The following reagents are supplied with this product:
Show all Collapse all NEB # Component Name Component # Stored at (°C) Amount Concentration E0542S -20 Quick Ligation™ Kit M2200SVIAL -20 1 x 0.03 ml Not Applicable Quick Ligation Reaction Buffer B2200SVIAL -20 1 x 1 ml 2 X Blunting Enzyme Mix M1201AVIAL -20 1 x 0.025 ml Not Applicable 10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X Deoxynucleotide Solution Mix (1 mM) N1201AVIAL -20 1 x 0.1 ml 1 mM
New England Biolabs (UK) Ltd
The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA Polymerase (NEB #M0203) which has both 3´ → 5´ exonuclease activity and 5´ → 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. To learn more about how to identify what type of overhang you have, visit this video tutorial.
Blunt Enzyme Mix supplied in: 100 mM KCl, 10 mM Tris-HCl(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% Triton X-100 and 50% Glycerol.
1X Blunting Buffer:
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl 2
0.025% Triton X-100
5 mM dithiothreitol
pH 7.5 at 25°C
Kit Components
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration E1201S -20 Blunting Enzyme Mix M1201AVIAL -20 1 x 0.025 ml Not Applicable 10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X Deoxynucleotide Solution Mix (1 mM) N1201AVIAL -20 1 x 0.1 ml 1 mM E1201L -20 Blunting Enzyme Mix M1201AAVIAL -20 1 x 0.125 ml Not Applicable 10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X Deoxynucleotide Solution Mix (1 mM) N1201AAVIAL -20 1 x 0.5 ml 1 mM
Advantages and Features
Application Features Prepare sheared, nebulized or restriction enzyme digested DNA for blunt-ended ligation into a plasmid, cosmid, fosmid or BAC vector Prepare PCR products for efficient blunt-end cloning
Properties & Usage
Related Products
Product Notes
New England Biolabs, Inc. Quick Blunting™ Kit – 20 reactions
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quick blunting kit
Multidimensional Application of Selenium Nanoparticles
Selenium is an essential trace element. It is a necessary dietary constituent of at least 25 human selenoproteins and enzymes containing selenocysteine. Additionally, as selenium is a semiconductor and photoelectrically active, it has more advanced applications such as xerography and
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs
E0542S E0542S
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
DNA Blunting Kit
Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).
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사람들이 주제에 대해 자주 검색하는 키워드 Quick Tip: Blunting Nails
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YouTube에서 quick blunting kit 주제의 다른 동영상 보기
주제에 대한 기사를 시청해 주셔서 감사합니다 Quick Tip: Blunting Nails | quick blunting kit, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.