당신은 주제를 찾고 있습니까 “quick blunting kit – Quick Tip: Blunting Nails“? 다음 카테고리의 웹사이트 Chewathai27.com/you 에서 귀하의 모든 질문에 답변해 드립니다: Chewathai27.com/you/blog. 바로 아래에서 답을 찾을 수 있습니다. 작성자 Bob Vila 이(가) 작성한 기사에는 조회수 2,662회 및 좋아요 16개 개의 좋아요가 있습니다.
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quick blunting kit 주제에 대한 동영상 보기
여기에서 이 주제에 대한 비디오를 시청하십시오. 주의 깊게 살펴보고 읽고 있는 내용에 대한 피드백을 제공하세요!
d여기에서 Quick Tip: Blunting Nails – quick blunting kit 주제에 대한 세부정보를 참조하세요
When nailing near the end of a piece of lumber, there’s a possibility of splitting the wood. Bob Vila explains how to minimize that problem.
quick blunting kit 주제에 대한 자세한 내용은 여기를 참조하세요.
Quick Blunting™ and Quick Ligation™ Kits – NEB
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into …
Source: www.nebiolabs.com.au
Date Published: 11/23/2022
View: 5492
Quick Blunting™ Kit #E1201 – NEB UK
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into …
Source: www.neb.uk.com
Date Published: 10/23/2021
View: 5040
New England Biolabs, Inc. Quick Blunting Kit – 20 reactions
The Quick Blunting Kit is used to convert DNA with incompatible 5′ or 3′ overhangs to 5′ phosphorylated, blunt-ended DNA for efficient blunt-end ligation …
Source: www.fishersci.com
Date Published: 12/25/2022
View: 3893
Quick blunting kit | Sigma-Aldrich
quick blunting kit … This cloning protocol includes selecting the cloning system and plasm vector, plasm restriction digestion, fragment restriction …
Source: www.sigmaaldrich.com
Date Published: 2/30/2021
View: 8369
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New …
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into …
Source: pr.vwr.com
Date Published: 11/12/2022
View: 6536
DNA Blunting Kit – Takara Bio
The DNA Blunting Kit allows the conversion of 3′ and 5′ overhangs to blunt or flush ends. This conversion is accomplished simultaneously by the 3’→5′ …
Source: www.takarabio.com
Date Published: 8/24/2022
View: 7779
Quick Blunting Kit – New England Biolabs GmbH
Quick Blunting Kit, Article Number: E1201.
Source: www.neb-online.de
Date Published: 7/11/2022
View: 8488
주제와 관련된 이미지 quick blunting kit
주제와 관련된 더 많은 사진을 참조하십시오 Quick Tip: Blunting Nails. 댓글에서 더 많은 관련 이미지를 보거나 필요한 경우 더 많은 관련 기사를 볼 수 있습니다.
주제에 대한 기사 평가 quick blunting kit
- Author: Bob Vila
- Views: 조회수 2,662회
- Likes: 좋아요 16개
- Date Published: 2011. 10. 28.
- Video Url link: https://www.youtube.com/watch?v=P2YRiUGg7CA
Quick Blunting™ and Quick Ligation™ Kits
Kit Components
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction.The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
The following reagents are supplied with this product:
Show all Collapse all NEB # Component Name Component # Stored at (°C) Amount Concentration E0542S -20 Quick Ligation™ Kit M2200SVIAL -20 1 x 0.03 ml Not Applicable Quick Ligation Reaction Buffer B2200SVIAL -20 1 x 1 ml 2 X Blunting Enzyme Mix M1201AVIAL -20 1 x 0.025 ml Not Applicable 10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X Deoxynucleotide Solution Mix (1 mM) N1201AVIAL -20 1 x 0.1 ml 1 mM
New England Biolabs (UK) Ltd
The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA Polymerase (NEB #M0203) which has both 3´ → 5´ exonuclease activity and 5´ → 3´ polymerase activity. T4 Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. To learn more about how to identify what type of overhang you have, visit this video tutorial.
Blunt Enzyme Mix supplied in: 100 mM KCl, 10 mM Tris-HCl(pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% Triton X-100 and 50% Glycerol.
1X Blunting Buffer:
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl 2
0.025% Triton X-100
5 mM dithiothreitol
pH 7.5 at 25°C
Kit Components
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration E1201S -20 Blunting Enzyme Mix M1201AVIAL -20 1 x 0.025 ml Not Applicable 10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X Deoxynucleotide Solution Mix (1 mM) N1201AVIAL -20 1 x 0.1 ml 1 mM E1201L -20 Blunting Enzyme Mix M1201AAVIAL -20 1 x 0.125 ml Not Applicable 10X Blunting Buffer B1201SVIAL -20 1 x 0.5 ml 10 X Deoxynucleotide Solution Mix (1 mM) N1201AAVIAL -20 1 x 0.5 ml 1 mM
Advantages and Features
Application Features Prepare sheared, nebulized or restriction enzyme digested DNA for blunt-ended ligation into a plasmid, cosmid, fosmid or BAC vector Prepare PCR products for efficient blunt-end cloning
Properties & Usage
Related Products
Product Notes
New England Biolabs, Inc. Quick Blunting™ Kit – 20 reactions
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quick blunting kit
Multidimensional Application of Selenium Nanoparticles
Selenium is an essential trace element. It is a necessary dietary constituent of at least 25 human selenoproteins and enzymes containing selenocysteine. Additionally, as selenium is a semiconductor and photoelectrically active, it has more advanced applications such as xerography and
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs
E0542S E0542S
QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs
Nucleic Acid Reagents Cloning Kits and Ligation
NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.
The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase which has both 3´→ 5´ exonuclease activity and 5´→ 3´ polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
DNA Blunting Kit
Takara Bio USA, Inc. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. Our mission is to develop high-quality innovative tools and services to accelerate discovery.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED).
키워드에 대한 정보 quick blunting kit
다음은 Bing에서 quick blunting kit 주제에 대한 검색 결과입니다. 필요한 경우 더 읽을 수 있습니다.
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사람들이 주제에 대해 자주 검색하는 키워드 Quick Tip: Blunting Nails
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YouTube에서 quick blunting kit 주제의 다른 동영상 보기
주제에 대한 기사를 시청해 주셔서 감사합니다 Quick Tip: Blunting Nails | quick blunting kit, 이 기사가 유용하다고 생각되면 공유하십시오, 매우 감사합니다.
